Jung Nam, Xia Chunlei, Jang Yong-Hyeok, Kim Hye-Won, Chung Yun Doo, Chon Tae-Soo
Research Institute of Computer, Information and Communication, Pusan National University, Busan 46241, Republic of Korea.
Research and Development, Ecology and Future Research Institute, Busan 46228, Republic of Korea.
Animals (Basel). 2025 May 22;15(11):1515. doi: 10.3390/ani15111515.
In the present study, groups of 10 adult males from wild-type strain Canton-S and corresponding mutant were continuously observed using automatic digitization. Data based on instantaneous movement and cumulated movement positions were obtained for micro-areas providing space for resources (food and moisture) and for activity (edge area and intermediate area [between edge and center-diffusion areas]) within the observation arena for 24 h. The results confirmed the natural tendency of local aggregation among individuals within the observation arena (14 cm × 14 cm) at a relatively low density of ten individuals. For Canton-S, temporal cooccurring patterns among different parameters were observed as time progressed, with two primary patterns identified in the resource supply areas: single peak and double peaks. The single peak was observed with maximum speed and -index, indicating minimum degree of isolated individuals from groups, during the transition from the photoperiod to the scotoperiod. The double peaks occurred before (mid-to-late photoperiod) and after (end of scotoperiod) the single peak, co-occurring temporally with a number of parameters including duration rates, stop number (total occurrence of pauses), stop time (total duration of pauses), mean crowding (MC), and social space index (SSI), indicating local aggregations for feeding in accordance with maximum durations in resource supply areas. Temporally cooccurring trends in parameters were also found with the stop number and SSI in micro-areas associated with activity, indicating that short pauses were needed to keep balance between attraction and repulsion between nearby individuals. Overall, the measured parameters varied depending on the micro-area, light phase, and strain. In particular, behavioral differences were observed for , including an increase in speed, especially in the areas related to activity during the scotoperiod. Between strains, behavioral differences in the measured parameters were observed less for than Canton-S.
在本研究中，使用自动数字化技术对来自野生型品系Canton-S和相应突变体的每组10只成年雄性个体进行了连续观察。在观察区域内，针对提供资源（食物和水分）空间的微区域以及活动区域（边缘区域和中间区域[边缘与中心扩散区域之间]），获取了基于瞬时运动和累积运动位置的数据，观察时长为24小时。结果证实了在观察区域（14厘米×14厘米）内，个体在相对较低的十只个体密度下自然存在局部聚集的趋势。对于Canton-S，随着时间的推移，观察到不同参数之间存在时间上的共现模式，在资源供应区域识别出两种主要模式：单峰和双峰。在从光周期向暗周期过渡期间，观察到单峰出现时速度和指数最大，表明个体与群体隔离的程度最小。双峰出现在单峰之前（光周期中后期）和之后（暗周期结束时），在时间上与包括持续时间率、停顿次数（停顿的总发生次数）、停顿时间（停顿的总持续时间）、平均拥挤度（MC）和社会空间指数（SSI）等多个参数同时出现，表明根据资源供应区域的最长持续时间进行局部聚集觅食。在与活动相关的微区域中，停顿次数和SSI也发现了参数在时间上的共现趋势，表明需要短暂停顿以保持附近个体之间吸引和排斥的平衡。总体而言，测量参数因微区域、光照阶段和品系而异。特别是，观察到[具体情况未明确给出]存在行为差异，包括速度增加，尤其是在暗周期期间与活动相关的区域。在品系之间，观察到[具体情况未明确给出]测量参数的行为差异比Canton-S少。
