Ma Jianfeng, Liang Shuang, Chen Siyu, Shi Yuqian, Zou Yu, Chen Lei, Niu Lili, Zhao Ye, Wang Yan, Shen Linyuan, Zhu Li, Gan Mailin
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Animals (Basel). 2025 Jun 1;15(11):1631. doi: 10.3390/ani15111631.
Extracellular vesicles (EVs) contain bioactive substances and mediate a multitude of physiological functions. EVs can be found in most body fluids and are particularly abundant in semen. EVs have the potential to become a biomarker for the quality of boar semen. In this study, EVs were isolated from the semen of relatively young (10 months of age, Y-EVs) and old (30 months of age, O-EVs) duroc boars using ultracentrifugation. The isolated EVs were characterized using a transmission electron microscope, nanoparticle tracking analysis, and Western blotting. MicroRNA (miRNA) profiles and metabolomes were analyzed using high-throughput sequencing and liquid chromatography-mass spectrometry, respectively. The median particle sizes of Y-EVs and O-EVs were 151.3 nm and 162.1 nm, respectively. miR-148a-3p, miR-10b, miR-21-5p, miR-10a-5p, let-7a, etc., were identified as highly enriched miRNAs in seminal EVs of boars. Comparative analysis revealed 41 differentially expressed miRNAs and 132 differential metabolites between Y-EVs and O-EVs. Notably, 18 miRNAs were upregulated in O-EVs, such as miR-339-5p, miR-125a, miR-423-3p, and miR-29c, which were mainly enriched in endocytosis, focal adhesion, and adherens junction. KEGG pathway analysis further indicated that differential metabolites were enriched in glycerophospholipid metabolism. These results provide an insight into the functional roles of seminal EVs.
细胞外囊泡（EVs）含有生物活性物质，并介导多种生理功能。EVs存在于大多数体液中，在精液中尤为丰富。EVs有潜力成为公猪精液质量的生物标志物。在本研究中，使用超速离心法从相对年轻（10月龄，Y-EVs）和年老（30月龄，O-EVs）的杜洛克公猪精液中分离出EVs。使用透射电子显微镜、纳米颗粒跟踪分析和蛋白质印迹法对分离出的EVs进行表征。分别使用高通量测序和液相色谱-质谱分析法分析微小RNA（miRNA）谱和代谢组。Y-EVs和O-EVs的中位粒径分别为151.3 nm和162.1 nm。miR-148a-3p、miR-10b、miR-21-5p、miR-10a-5p、let-7a等被鉴定为公猪精液EVs中高度富集的miRNA。比较分析显示Y-EVs和O-EVs之间有41个差异表达的miRNA和132种差异代谢物。值得注意的是，18种miRNA在O-EVs中上调，如miR-339-5p、miR-125a、miR-423-3p和miR-29c，它们主要富集在内吞作用、粘着斑和紧密连接中。KEGG通路分析进一步表明差异代谢物富集在甘油磷脂代谢中。这些结果为精液EVs的功能作用提供了见解。
Zotero 插件
