Zhu Minyi, Ma Minhui, Chen Shuo, Yuan Rongfang, Wang Shaona
Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Molecules. 2025 May 30;30(11):2412. doi: 10.3390/molecules30112412.
Pollution caused by N and P is a significant contributor to water eutrophication. While traditional biological treatment processes can remove some N and P elements from water, the effluent quality often fails to meet the stringent requirements of sensitive areas. The autotrophic denitrification's simultaneous nitrogen and phosphorus removal pro-cess, known for its low operating cost and minimal sludge production, has garnered considerable attention from researchers. In this study, natural pyrite was used for the removal of nitrogen and phosphorus in a denitrification system, and the underlying mechanisms were elucidated. The results indicate that the N and P removal efficiency was influenced by empty bed contact time (EBCT) and the pH value. The highest NO-N removal rate of 90.24% was achieved at an EBCT of 8 h, while the PO-P removal rate reached 81.58% at an EBCT of 12 h. The addition of a carbon source enhanced the synergistic autotrophic/heterotrophic denitrification, significantly improving phosphorus removal with an increasing C/N ratio. Microbial characteristics analysis revealed that, at the phylum level, , , and played a crucial role in heterotrophic autotrophic denitrification. At the genus level, , , and were key players in heterotrophic denitrification, while , , and were associated with autotrophic denitrification.
氮和磷造成的污染是水体富营养化的一个重要因素。虽然传统的生物处理工艺可以从水中去除一些氮和磷元素，但出水水质往往无法满足敏感区域的严格要求。自养反硝化同步脱氮除磷工艺以其运行成本低、污泥产量少而受到研究人员的广泛关注。在本研究中，天然黄铁矿用于反硝化系统中氮和磷的去除，并阐明了其潜在机制。结果表明，氮和磷的去除效率受空床接触时间（EBCT）和pH值的影响。在EBCT为8 h时，NO-N去除率最高可达90.24%，而在EBCT为12 h时，PO-P去除率达到81.58%。碳源的添加增强了自养/异养反硝化的协同作用，随着C/N比的增加，显著提高了磷的去除率。微生物特征分析表明，在门水平上， 、 和 在异养自养反硝化中起关键作用。在属水平上， 、 和 是异养反硝化的关键参与者，而 、 和 与自养反硝化有关。
