Fernandes Beatriz, Silva Margarida, Alves Susana Paula, Schmidt Vanessa Merta, Bizarro Ana Filipa, Pinto Marta, Pereira Hugo, Marto Joana, Lourenço Ana Mafalda
Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Vila Real, Portugal.
Vet Dermatol. 2025 Aug;36(4):462-473. doi: 10.1111/vde.13363. Epub 2025 Jun 12.
Canine atopic dermatitis (cAD) is a complex skin disease characterised by barrier dysfunction. Studies regarding the role of skin surface lipids (SSL) in cAD are needed.
OBJECTIVES/HYPOTHESIS: Evaluate the feasibility of using D-squame tape-stripping for SSL collection and ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) for untargeted lipidomic analysis. A secondary objective was to identify significant differences in SSL between atopic and healthy dogs, and between different body sites.
Sixteen client-owned Labrador retrievers, eight atopic and eight healthy were recruited through vaccination or dermatology appointments.
Skin samples were collected from three body sites (thigh, interdigital and inguinal) using D-Squame tapes. Untargeted lipidomic analysis was conducted using UHPLC-HRMS, and data were processed with MS-DIAL and LipidSearch software.
This study identified 114 SSLs, predominantly ceramides (66.2%) and diacylglycerols (30.5%). The percentage of lipid classes and ceramide subclasses did not significantly differ between healthy and atopic dogs. Two ceramide and two triacylglycerol species were significantly higher in atopic dogs, while another two ceramide species were significantly lower. Additionally, notable regional differences in lipid profiles were observed.
Our findings suggest that D-squame tape-stripping combined with UHPLC-HRMS is a feasible method for SSL research in cAD. Lipid species-specific differences and significant regional variations were found, emphasising the importance of considering body sites in future studies. This study underscores the need for further research to understand the role of SSL in cAD and the insights that untargeted lipidomic analysis can provide.
犬特应性皮炎（cAD）是一种以屏障功能障碍为特征的复杂皮肤病。需要开展关于皮肤表面脂质（SSL）在cAD中作用的研究。
目的/假设：评估使用D-皮肤角质层胶带剥离法收集SSL以及使用超高效液相色谱-高分辨率质谱联用技术（UHPLC-HRMS）进行非靶向脂质组学分析的可行性。第二个目标是确定特应性犬与健康犬之间以及不同身体部位之间SSL的显著差异。
通过疫苗接种或皮肤科预约招募了16只客户拥有的拉布拉多猎犬，其中8只为特应性犬，8只为健康犬。
使用D-皮肤角质层胶带从三个身体部位（大腿、趾间和腹股沟）采集皮肤样本。使用UHPLC-HRMS进行非靶向脂质组学分析，并使用MS-DIAL和LipidSearch软件处理数据。
本研究鉴定出114种SSL，主要为神经酰胺（66.2%）和二酰基甘油（30.5%）。健康犬和特应性犬之间脂质类别和神经酰胺亚类的百分比没有显著差异。特应性犬中有两种神经酰胺和两种三酰基甘油种类显著更高，而另外两种神经酰胺种类显著更低。此外，观察到脂质谱存在显著的区域差异。
我们的研究结果表明，D-皮肤角质层胶带剥离法结合UHPLC-HRMS是cAD中SSL研究的一种可行方法。发现了脂质种类特异性差异和显著的区域差异，强调在未来研究中考虑身体部位的重要性。本研究强调需要进一步开展研究以了解SSL在cAD中的作用以及非靶向脂质组学分析所能提供的见解。
