Oshima Naoki, Ishimura Norihisa, Kotani Satoshi, Yazaki Tomotaka, Oka Akihiko, Fukuba Nobuhiko, Mishima Yoshiyuki, Tobita Hiroshi, Shibagaki Kotaro, Kawashima Kousaku, Kinoshita Yoshikazu, Ishihara Shunji
Department of Internal Medicine II, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
Inflammatory Bowel Disease Center, Shimane University Hospital, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
J Clin Biochem Nutr. 2025 May;76(3):289-295. doi: 10.3164/jcbn.24-193. Epub 2024 Dec 28.
The number of eosinophilic esophagitis (EoE) patients has been rapidly increasing worldwide in recent years, though remains relatively lower in Japan. To determine whether Japanese EoE cases have pathogenic differences, microRNA (miRNA) transcriptome analyses of esophageal biopsy specimens from patients with EoE were performed to identify involved miRNAs and the results were compared with those for cases reported in Western countries. Japanese patients with EoE ( = 9) or reflux esophagitis (RE) ( = 4) were enrolled, with the latter serving as controls. Biopsies of esophageal mucosal tissue were performed as a part of an upper gastrointestinal endoscopy, followed by comprehensive miRNA expression analysis (2,588 target human miRNAs) using microarray technology. The EoE patients were then treated with a proton pomp inhibitor, and miRNA expression in the esophagus was compared before and after treatment. Hierarchical cluster analysis findings showed clear differences in miRNA expression patterns between the EoE and RE patients, with increased expression of 13 and decreased expression of 10 miRNAs noted in the EoE cases. These results were then compared with miRNA expression in esophageal mucosa of EoE patients presented in Western reports and the expression profiles were found to be very similar. In addition, changes in expression profiles of several miRNAs before and after treatment were observed in the present EoE patients. miRNA microarray analysis of Japanese EoE patients demonstrated a significant overlap of miRNA expression in comparison with Western patients, indicating that EoE likely represents the same disease among diverse racial populations and shows a consistent pathogenesis worldwide.
近年来，嗜酸性粒细胞性食管炎（EoE）患者数量在全球范围内迅速增加，不过在日本该数量仍相对较少。为确定日本EoE病例是否存在致病差异，对EoE患者的食管活检标本进行了微小RNA（miRNA）转录组分析，以鉴定相关miRNA，并将结果与西方国家报告的病例结果进行比较。纳入了日本EoE患者（n = 9）或反流性食管炎（RE）患者（n = 4），后者作为对照。作为上消化道内镜检查的一部分，对食管黏膜组织进行活检，随后使用微阵列技术进行全面的miRNA表达分析（2588种靶向人类miRNA）。然后对EoE患者使用质子泵抑制剂进行治疗，并比较治疗前后食管中的miRNA表达。层次聚类分析结果显示，EoE患者和RE患者之间的miRNA表达模式存在明显差异，EoE病例中有13种miRNA表达增加，10种miRNA表达减少。然后将这些结果与西方报告中EoE患者食管黏膜中的miRNA表达进行比较，发现表达谱非常相似。此外，在本研究的EoE患者中观察到了几种miRNA治疗前后表达谱的变化。对日本EoE患者的miRNA微阵列分析表明，与西方患者相比，miRNA表达存在显著重叠，这表明EoE可能在不同种族人群中代表同一种疾病，并且在全球范围内具有一致的发病机制。
