Sukhavattanakul Pongpat, Rodthongkum Nadnudda, Ummartyotin Sarute
Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathumtani, Thailand.
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Food Chem X. 2025 May 26;28:102578. doi: 10.1016/j.fochx.2025.102578. eCollection 2025 May.
This study introduces a novel approach for sensing histamine by developing sensors from cellulose-based newspaper waste. The cellulose-based sensors, featuring diamine oxidase (DAO) as the sensing agent in cooperation with horseradish peroxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) solutions, are designed and assembled for targeted histamine detection. Experimental results demonstrate the sensor's exceptional sensitivity, with the observed color change serving as a reliable indicator of histamine concentration. In the presence of peroxidase and HO, the hydroxypropyl methylcellulose (HPMC)/DAO-immobilized enzyme-hydrolyzed cellulose-based film sensor gets oxidized, resulting in a blue spot. The highest sensitivity to histamine (3.30 mg/mL) was exhibited by the 3 /w % HPMC/DAO-immobilized enzyme-hydrolyzed cellulose-based film, which showed the lowest b* value (-8.85 ± 0.01). This study showcases the potential of these films as an effective and cost-efficient platform for histamine detection in fermented beverages, offering promising applications in food safety screening. Ultimately, the research underscores the environmental sustainability of the HPMC-based hybrid material.
本研究介绍了一种通过利用基于纤维素的废旧报纸开发传感器来检测组胺的新方法。基于纤维素的传感器以二胺氧化酶（DAO）作为传感剂，与辣根过氧化物酶（HRP）和3,3',5,5'-四甲基联苯胺（TMB）溶液协同作用，设计并组装用于靶向组胺检测。实验结果证明了该传感器具有卓越的灵敏度，观察到的颜色变化可作为组胺浓度的可靠指标。在过氧化物酶和过氧化氢存在的情况下，固定有羟丙基甲基纤维素（HPMC）/DAO的酶水解纤维素基薄膜传感器被氧化，产生蓝色斑点。3%（质量分数）HPMC/DAO固定化酶水解纤维素基薄膜对组胺的灵敏度最高（3.30 mg/mL），其b*值最低（-8.85±0.01）。本研究展示了这些薄膜作为发酵饮料中组胺检测的有效且经济高效平台的潜力，在食品安全筛查中具有广阔的应用前景。最终，该研究强调了基于HPMC的混合材料的环境可持续性。
