用于在小鼠皮肤或大脑中可视化蜱传黄病毒的免疫荧光测定法。

Immunofluorescence Assays to Visualize Tick-Borne Flaviviruses in Mouse Skin or Brain.

作者信息

Santos Rodrigo I, Hermance Meghan E

机构信息

Jerry M. Wallace School of Osteopathic Medicine, Campbell University, Buies Creek, NC, USA.

Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL, USA.

出版信息

Methods Mol Biol. 2025;2936:85-97. doi: 10.1007/978-1-0716-4587-1_10.

DOI:10.1007/978-1-0716-4587-1_10

PMID:40512312

Abstract

Multiplex immunofluorescence (IF) utilizes a combination of different fluorophore-linked antibodies to facilitate the simultaneous observation of multiple antigenic epitopes within a tissue section. In this chapter, we describe how tick-borne flaviviruses can be visualized in mouse skin biopsies harvested from the tick feeding site or in mouse brain tissue. Using antibodies specific to the North American tick-borne flavivirus, Powassan virus (POWV), and to Mus musculus cell markers, IF multiplex labeling experiments allow us to visualize how POWV antigen co-localizes with host cell markers. Such IF multiplexing in mouse skin and brain tissue suggests which host cells are targets of viral infection.

摘要

多重免疫荧光（IF）利用不同荧光团连接抗体的组合，以便在组织切片中同时观察多个抗原表位。在本章中，我们描述了如何在从蜱叮咬部位采集的小鼠皮肤活检组织或小鼠脑组织中观察蜱传黄病毒。使用针对北美蜱传黄病毒波瓦桑病毒（POWV）和小家鼠细胞标志物的抗体，IF多重标记实验使我们能够观察POWV抗原如何与宿主细胞标志物共定位。在小鼠皮肤和脑组织中进行的这种IF多重检测表明哪些宿主细胞是病毒感染的靶标。