Velo-Suarez Lourdes, Moalic Yann, Guilloux Charles-Antoine, Ame Jacky, Lamoureux Claudie, Gouriou Stéphanie, Le Berre Rozenn, Beauruelle Clémence, Héry-Arnaud Geneviève
Center of Biome Analysis and Microbiota (CBAM), Brest University Hospital, Brest, France.
INSERM, EFS, UMR 1078 Inserm Unit UMR1078 'Genetics, Genomics and Biotechnology' BIGG team Microbiota axis, Faculty of Medicine, GGB, Brest University, Brest, France.
Microb Genom. 2025 Jun;11(6). doi: 10.1099/mgen.0.001411.
Data on the genomics of species other than (POTG), particularly within pulmonary environments, are scarce. In this study, we conducted whole-genome sequencing on pulmonary isolates of POTG, specifically (=3), (=1) and (=2), from people with cystic fibrosis. These genomic analyses were complemented with antimicrobial susceptibility tests for these strains. We compared the genomic sequences of these pulmonary isolates with those of previously characterized species. Our study revealed a distinct clade differentiation between non-pigmented and pigmented species. Unlike , the pulmonary POTG strains lacked known virulence genes, with the exception of a putative haemolysin gene. Regarding antibiotic resistance, notable resistances were limited to vancomycin in and clindamycin in . These findings support the hypothesis that POTG species may predominantly behave as commensals in the lung environment rather than as pathogens.
除（POTG）之外的其他物种的基因组学数据，尤其是肺部环境中的数据很少。在本研究中，我们对来自囊性纤维化患者的POTG肺部分离株进行了全基因组测序，具体为（=3）、（=1）和（=2）。这些基因组分析辅以对这些菌株的抗菌药敏试验。我们将这些肺部分离株的基因组序列与先前鉴定的物种的序列进行了比较。我们的研究揭示了非色素沉着和色素沉着物种之间明显的进化枝分化。与不同，肺部POTG菌株除了一个假定的溶血素基因外，缺乏已知的毒力基因。关于抗生素耐药性，显著的耐药性仅限于中的万古霉素和中的克林霉素。这些发现支持了POTG物种在肺部环境中可能主要表现为共生菌而非病原体的假设。
