Ganz T, Sherman M P, Selsted M E, Lehrer R I
Am Rev Respir Dis. 1985 Oct;132(4):901-4. doi: 10.1164/arrd.1985.132.4.901.
Adult rabbit alveolar macrophages (AM) contain 2 cationic peptides with a broad spectrum of antimicrobial activity in vitro against bacteria, fungi, and some enveloped viruses. We determined the amounts of both peptides qualitatively in 1-day-old (1d), 7-day-old (7d), 21-day-old (21d), and adult rabbit AM and found that 1d AM were deficient in both peptides. The levels of MCP-1 extractable from AM were quantitated relative to known standards of purified peptides and were found to increase 6-fold between 1d and 21d AM. Adult AM yielded 9 times as much MCP-1 as did 1d AM despite nearly the same acid-extractable protein content per cell. Using immunoperoxidase techniques we showed that the deficiency of MCP-1 and MCP-2 involves 1d AM uniformly and that all AM 7 days or older have detectable MCP. Seven-day-old AM (and to a lesser extent 1d AM) incorporated 35S-cysteine into intracellular MCP in cell culture, indicating that AM actively synthesize these peptides. The deficiency of these antimicrobial substances may contribute to functional immaturity of newborn rabbit AM.
成年兔肺泡巨噬细胞（AM）含有两种阳离子肽，在体外对细菌、真菌和一些包膜病毒具有广谱抗菌活性。我们定性测定了1日龄（1d）、7日龄（7d）、21日龄（21d）和成年兔AM中这两种肽的含量，发现1d AM这两种肽均缺乏。相对于纯化肽的已知标准品，定量测定了从AM中可提取的MCP-1水平，发现其在1d和21d AM之间增加了6倍。尽管每个细胞的酸可提取蛋白含量几乎相同，但成年AM产生的MCP-1是1d AM的9倍。使用免疫过氧化物酶技术，我们表明MCP-1和MCP-2的缺乏在1d AM中是一致的，并且所有7日龄或更大的AM都有可检测到的MCP。7日龄的AM（1d AM程度较轻）在细胞培养中将35S-半胱氨酸掺入细胞内MCP中，表明AM可积极合成这些肽。这些抗菌物质的缺乏可能导致新生兔AM功能不成熟。
