Zeligs B J, Nerurkar L S, Bellanti J A
Pediatr Res. 1977 Dec;11(12):1208-11. doi: 10.1203/00006450-197712000-00008.
Phagocytic and bactericidal function of rabbit alveolar macrophages (AMs) lavaged from animals during the course of postnatal maturation was studied. Staphylococcus aureus and a temperature-sensitive mutant of Escherichia coli, which could not replicate at 37 degrees during the functional assays, were employed as test bacteria. Assays of the phagocytic capacity of AMs from rabbits of various age groups revealed no significant differences either in the percentage of AMs which took up bacteria (79-90%) or in the number of bacteria taken up per AM (Table 1). In contrast, bactericidal activity of AMs was found to increase with increasing animal age. No bactericidal activity was detected in AMs from newborn animals (Figs. 1 and 2), whereas AMs from 7-day-old animals exhibited at least a bacteristatic activity against S. aureus (Fig. 1) and AMs from 28-day-old rabbits showed marked bactericidal activity, essentially the same as that of AMs from adult rabbits. Adult AMs killed 75% of the S. aureus and 60% of the E. coli within 120 min (Figs. 1 and 2).
研究了从出生后成熟过程中的动物体内灌洗出的兔肺泡巨噬细胞（AMs）的吞噬和杀菌功能。金黄色葡萄球菌和一种在功能测定期间于37摄氏度无法复制的大肠杆菌温度敏感突变体被用作测试细菌。对来自不同年龄组兔子的AMs吞噬能力的测定显示，摄取细菌的AMs百分比（79 - 90%）或每个AMs摄取的细菌数量均无显著差异（表1）。相比之下，发现AMs的杀菌活性随动物年龄增长而增加。在新生动物的AMs中未检测到杀菌活性（图1和图2），而7日龄动物的AMs对金黄色葡萄球菌至少表现出抑菌活性（图1），28日龄兔子的AMs显示出显著的杀菌活性，与成年兔子的AMs基本相同。成年AMs在120分钟内杀死了75%的金黄色葡萄球菌和60%的大肠杆菌（图1和图2）。
