Fillion G, Fillion M P, Rousselle J C, Beaudoin D, Goiny M, Jacob J
C R Acad Hebd Seances Acad Sci D. 1977 Mar 14;284(11):979-81.
5 hydroxytryptamine binds to crude brain membrane preparations with two different affinities (KD = 1 to 2 X 10(-9) M for the highest, 1 to 2 X 10(-8) M for the lowest). LSD also binds with two affinities (KD = 3 to 4 X 10(-9) M and KD = 2 to 3 X 10(-8) M). Subcellular distribution of these sites shows that binding involves the two binding affinities in microsomal membranes but solely the high affinity binding sites are present in purified synaptosomal membranes. High affinity sites for 5 HT and for LSD are different as no direct competitive inhibition is observed in that case. On microsomal membranes, direct relationship occurs between low affinity binding for 5 HT and high affinity binding for LSD.
5-羟色胺以两种不同亲和力与粗制脑膜制剂结合（最高亲和力时KD = 1至2×10⁻⁹ M，最低亲和力时KD = 1至2×10⁻⁸ M）。麦角酸二乙酰胺（LSD）也以两种亲和力结合（KD = 3至4×10⁻⁹ M和KD = 2至3×10⁻⁸ M）。这些结合位点的亚细胞分布表明，微粒体膜中的结合涉及两种结合亲和力，但纯化的突触体膜中仅存在高亲和力结合位点。5-羟色胺（5-HT）和LSD的高亲和力位点不同，因为在这种情况下未观察到直接竞争抑制作用。在微粒体膜上，5-HT的低亲和力结合与LSD的高亲和力结合之间存在直接关系。
