Xu Jie, Zheng Chunfu, Wang Yiqiang
Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian Province, China.
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2025;2940:379-386. doi: 10.1007/978-1-0716-4615-1_33.
Systematic evolution of ligands by exponential enrichment (SELEX) utilizes multiple rounds of binding of a combinatorial oligo(deoxy)nucleotide library to generate high-affinity DNA/RNA ligands for the studied molecules. Common applications of SELEX include developing aptamers for diagnosis or therapeutics in translational field or studying DNA/RNA interactions with partners in basic research fields. The protocol described here was used to characterize the features of DNA segments bound by NLRP3 proteins, which were recently discovered to translocate into nuclei upon viral infection of cells. These results validated the hypothesis that the nuclear-resident NLRP3 protein functions as a transcription factor that affects transcription.
指数富集的配体系统进化技术（SELEX）利用组合寡聚（脱氧）核苷酸文库的多轮结合来生成针对所研究分子的高亲和力DNA/RNA配体。SELEX的常见应用包括在转化领域开发用于诊断或治疗的适配体，或在基础研究领域研究DNA/RNA与其他分子的相互作用。此处描述的实验方案用于表征与NLRP3蛋白结合的DNA片段的特征，最近发现这些片段在细胞受到病毒感染时会转移到细胞核中。这些结果验证了一个假设，即驻留在细胞核中的NLRP3蛋白作为一种影响转录的转录因子发挥作用。
