Moriwaki Taijun, Tani Hidenori, Haga Kotaro, Tohyama Shugo
Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan.
Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.
STAR Protoc. 2025 Jun 20;6(2):103891. doi: 10.1016/j.xpro.2025.103891. Epub 2025 Jun 12.
Three-dimensional cultures mimic in vivo environments better than two-dimensional cultures and are often used in drug discovery research. Herein, we present a protocol for producing homogeneous induced pluripotent stem cell (iPSC) spheroids and microtissues using the suction technique. We describe steps for preparing the suction device, preparing and seeding cells, and suction sedimentation of cells. We then detail procedures for self-assembly and evaluation of spheroids. For complete details on the use and execution of this protocol, please refer to Moriwaki et al..
三维培养比二维培养能更好地模拟体内环境，常用于药物研发研究。在此，我们介绍一种使用抽吸技术生产均匀诱导多能干细胞（iPSC）球体和微组织的方案。我们描述了制备抽吸装置、制备和接种细胞以及细胞抽吸沉降的步骤。然后详细说明了球体的自组装和评估程序。有关本方案使用和执行的完整详细信息，请参考森胁等人的研究。
