Zhong Jie, Bai Xin Yi, Li Xiao Gang, Zhu Jun Zi
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China.
Int J Food Microbiol. 2025 Oct 2;441:111319. doi: 10.1016/j.ijfoodmicro.2025.111319. Epub 2025 Jun 11.
Colletotrichum scovillei is a pathogen that causes anthracnose, resulting in significant postharvest losses in pepper fruit. Biocontrol using antagonistic microbes is a promising solution for sustainable disease management. We previously identified Streptomyces olivoreticuli ZZ-21, which has broad-spectrum antifungal activity. In this study, we evaluated the antagonistic ability of ZZ-21 specifically against C. scovillei in both in vitro and pepper fruit assays, as well as the possible mechanisms involved. Dual culture assays revealed that strain ZZ-21 achieved 86.25 % mycelial inhibition rate against C. scovillei. Pepper fruit trials indicated that the culture filtrate of ZZ-21 significantly reduced both the incidence and lesion size of anthracnose caused by C. scovillei, while maintaining fruit quality. The efficacy of control was dependent on the concentrations of the ZZ-21 culture filtrate, with a 100 % control efficiency observed in the treatment utilizing 100 % culture filtrate. Moreover, the ZZ-21 culture filtrate obviously inhibited spore germination and damaged the cell membrane permeability of C. scovillei, while also inducing plant resistance by enhancing the activities of defense-related enzymes. The primary inhibitory substances were included in proteins and n-butyl alcohol crude extracts of the ZZ-21 culture filtrate, which exhibited EC values of 56.88 ± 0.82 μg/mL and 65.37 ± 0.36 μg/mL, respectively. These substances were analyzed using LC-MS and were thought to contribute to the broad-spectrum antifungal activity of ZZ-21. Hence, strain ZZ-21 is a potent biocontrol agent for controlling anthracnose in pepper caused by C. scovillei.
斯氏炭疽菌是一种可引发炭疽病的病原菌，会导致辣椒果实采后出现严重损失。利用拮抗微生物进行生物防治是可持续病害管理的一个有前景的解决方案。我们之前鉴定出了具有广谱抗真菌活性的橄榄网状链霉菌ZZ-21。在本研究中，我们在体外试验和辣椒果实试验中评估了ZZ-21对斯氏炭疽菌的拮抗能力以及可能涉及的机制。对峙培养试验表明，菌株ZZ-21对斯氏炭疽菌的菌丝抑制率达到了86.25%。辣椒果实试验表明，ZZ-21的发酵滤液显著降低了由斯氏炭疽菌引起的炭疽病的发病率和病斑大小，同时保持了果实品质。防治效果取决于ZZ-21发酵滤液的浓度，在使用100%发酵滤液的处理中观察到100%的防治效率。此外，ZZ-21发酵滤液明显抑制了斯氏炭疽菌的孢子萌发并破坏了其细胞膜通透性，同时还通过增强防御相关酶的活性诱导植物抗性。主要抑制物质包括在ZZ-21发酵滤液的蛋白质和正丁醇粗提物中，其EC值分别为56.88±0.82μg/mL和65.37±0.36μg/mL。使用液相色谱-质谱联用仪对这些物质进行了分析，认为它们有助于ZZ-21的广谱抗真菌活性。因此，菌株ZZ-21是防治由斯氏炭疽菌引起的辣椒炭疽病的一种有效的生物防治剂。
