Luo Wen, Wu Hongling, Huang Ying, Ye Kun, Zou Wenzheng, Han Fang
State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361000, PR China.
State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361000, PR China.
Fish Shellfish Immunol. 2025 Oct;165:110498. doi: 10.1016/j.fsi.2025.110498. Epub 2025 Jun 13.
Viperin (virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible), also known as RSAD2 (radical SAM domain-containing 2), is one of the interferon-stimulated genes and plays an important role in cellular antiviral response. In this study, the viperin gene in large yellow croaker (Larimichthys crocea), designated LcViperin, was identified and characterized by investigating its tissue expression pattern and transcriptional response to viral infection and analyzing the differentially expressed genes (DEGs) upon overexpression of LcViperin in LCM10 cells revealed by RNA-seq transcriptome profiling. The results showed that LcViperin was highly expressed in the blood and organs of the immune system such as spleen and kidney, and the LcViperin protein was localized to the endoplasmic reticulum. The transcript level of LcViperin can be upregulated by large yellow croaker iridovirus (LYCIV) infection and the simulated viral infection of poly (I: C). GO and KEGG analysis of the DEGs demonstrated that the overexpression of LcViperin activated macrophage endocytosis pathway and other pathways to eliminate the viral particles. Flow cytometry analysis indicated an increase in macrophage phagocytotic activity and an induced respiratory burst in LCM10 cells upon exposure to LcViperin protein. The transcriptome analysis results would help to understand the host immune response and the potential mechanism of fish Viperin protein in response to pathogen stimulation.
蝰蛇毒蛋白（病毒抑制蛋白，内质网相关，干扰素诱导），也称为RSAD2（含自由基SAM结构域的2），是干扰素刺激基因之一，在细胞抗病毒反应中起重要作用。在本研究中，通过研究大黄鱼（Larimichthys crocea）中的蝰蛇毒蛋白基因（命名为LcViperin）的组织表达模式和对病毒感染的转录反应，并分析RNA-seq转录组分析揭示的LcViperin在LCM10细胞中过表达后的差异表达基因（DEG），对其进行了鉴定和表征。结果表明，LcViperin在血液和免疫系统器官如脾脏和肾脏中高表达，且LcViperin蛋白定位于内质网。大黄鱼虹彩病毒（LYCIV）感染和聚（I：C）模拟病毒感染可上调LcViperin的转录水平。对DEG的GO和KEGG分析表明，LcViperin的过表达激活了巨噬细胞内吞途径和其他途径以清除病毒颗粒。流式细胞术分析表明，暴露于LcViperin蛋白后，LCM10细胞中巨噬细胞吞噬活性增加且诱导了呼吸爆发。转录组分析结果将有助于了解宿主免疫反应以及鱼类蝰蛇毒蛋白响应病原体刺激的潜在机制。
