Yuzhuo Wei, Li Liu, Shu Bu, Jing Yan, Yongqi Wang, Zhiwei Miao, Yi Xu
Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, China.
Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, China.
J Gastroenterol Hepatol. 2025 Aug;40(8):1991-2006. doi: 10.1111/jgh.17034. Epub 2025 Jun 15.
Sanguisorba officinalis L. and Sophora japonica L. (SOSJ) have been frequently used as medicinal pairs for treating ulcerative colitis (UC) due to their hemostatic properties. However, the mechanisms underlying their therapeutic effects remain unclear. This study aims to predict the targets of SOSJ for UC treatment using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QTOF-MS) and network pharmacology.
The efficacy of SOSJ was evaluated using a 3% dextrose sodium sulfate (DSS)-induced UC mice model. The general condition of the mice, histopathology, and expression of colonic inflammatory factors were assessed. Additionally, the effect of SOSJ on angiogenesis was evaluated by detecting the mRNA of colonic angiogenesis-related mediators, measuring microvessel density, and using transmission electron microscopy.
SOSJ significantly attenuated inflammation and inhibited pathological angiogenesis in UC mice. It alleviated weight loss, colon shortening, and the presence of pus and blood in stools. SOSJ also reduced the mRNA expression levels of IL-6, IL-1β, TNF-α, VEGF, VCAM1, Ang1, MMP1, MMP2, and MMP9. Furthermore, SOSJ decreased the protein expression of the PI3K-Akt pathway, an effect that could be reversed by 740Y-P, a specific PI3K activator.
S. officinalis L. and S. japonica L. exert therapeutic effects on mice with ulcerative colitis, potentially through the modulation of angiogenesis and the PI3K-Akt signalling pathway.
地榆和槐花（SOSJ）因其止血特性，常被用作治疗溃疡性结肠炎（UC）的药对。然而，其治疗作用的潜在机制仍不清楚。本研究旨在利用液相色谱 - 四极杆飞行时间串联质谱（LC - QTOF - MS）和网络药理学预测SOSJ治疗UC的靶点。
使用3%葡聚糖硫酸钠（DSS）诱导的UC小鼠模型评估SOSJ的疗效。评估小鼠的一般状况、组织病理学以及结肠炎症因子的表达。此外，通过检测结肠血管生成相关介质的mRNA、测量微血管密度以及使用透射电子显微镜来评估SOSJ对血管生成的影响。
SOSJ显著减轻UC小鼠的炎症并抑制病理性血管生成。它减轻了体重减轻、结肠缩短以及粪便中脓血的出现。SOSJ还降低了IL - 6、IL - 1β、TNF - α、VEGF、VCAM1、Ang1、MMP1、MMP2和MMP9的mRNA表达水平。此外，SOSJ降低了PI3K - Akt通路的蛋白表达，这一作用可被特异性PI3K激活剂740Y - P逆转。
地榆和槐花对溃疡性结肠炎小鼠具有治疗作用，可能是通过调节血管生成和PI3K - Akt信号通路实现的。
