Moalic Yann, Nguyen Toan Bao Hung, Hartunians Jordan, Birien Tiphaine, Thiel Axel, Jebbar Mohamed
Univ Brest, Ifremer, CNRS, EMR 6002 BIOMEX, BEEP, Plouzané, France.
Front Microbiol. 2025 May 30;16:1593936. doi: 10.3389/fmicb.2025.1593936. eCollection 2025.
In , energy metabolism genes are regulated by the sulfur-responsive transcriptional regulator SurR. In the piezophilic archaeon , these genes are also influenced by hydrostatic pressure. To explore the interaction between pressure, sulfur availability, and gene regulation, we constructed and analyzed several deletion mutants, including a partial knockout, under varying conditions. Our results show that hydrostatic pressure modulates the expression of energy metabolism genes and that SurR is essential for activating the hydrogenogenic gene cluster, even in sulfur-rich environments. Under sulfur limitation, the membrane-bound sulfur-reducing complex (MBS) is required for normal growth. These regulatory patterns expand current models derived from non-piezophilic species such as and . Overall, our findings indicate that hydrostatic pressure shapes SurR function in , reflecting its adaptive plasticity in extreme environments.
在[具体物种名称未给出]中，能量代谢基因受硫响应转录调节因子SurR调控。在嗜压古菌[具体物种名称未给出]中，这些基因也受静水压力影响。为探究压力、硫可用性与基因调控之间的相互作用，我们构建并分析了几个缺失突变体，包括一个部分敲除突变体，实验条件各不相同。我们的结果表明，静水压力调节能量代谢基因的表达，并且SurR对于激活产氢基因簇至关重要，即使在硫丰富的环境中也是如此。在硫限制条件下，正常生长需要膜结合硫还原复合物（MBS）。这些调控模式扩展了源自非嗜压物种如[具体物种名称未给出]和[具体物种名称未给出]的现有模型。总体而言，我们的研究结果表明，静水压力塑造了[具体物种名称未给出]中SurR的功能，反映了其在极端环境中的适应性可塑性。
