Klaude M, von der Decken A
Biochem Pharmacol. 1985 Oct 15;34(20):3627-31. doi: 10.1016/0006-2952(85)90222-9.
The effect of methionine-cysteine deficiency on the methylation of DNA purines by dimethylnitrosamine metabolites was studied in subadult and adult mice. In liver, no dietary effect on the specific methylation of 7-methylguanine was observed, while that of 3-methyladenine decreased in the adult animals. The specific methylation of guanine in the 0(6)-position and the ratio of 0(6)-methylguanine to 7-methylguanine increased significantly after methionine-cysteine deficiency. Methylation in kidney decreased in subadult but increased in adult mice. In lung, the amount of 7-methylguanine was significantly elevated after methionine-cysteine deficiency in both the subadult and adult mice. The results demonstrate an increase in the specific methylation in liver of guanine in the 0(6)-position by the methionine-cysteine deficient diet, together with differences in the methylation pattern between organs of the two age groups.
在未成年和成年小鼠中研究了蛋氨酸 - 半胱氨酸缺乏对二甲基亚硝胺代谢产物使DNA嘌呤甲基化的影响。在肝脏中，未观察到饮食对7 - 甲基鸟嘌呤特异性甲基化的影响，而成年动物中3 - 甲基腺嘌呤的特异性甲基化减少。蛋氨酸 - 半胱氨酸缺乏后，鸟嘌呤在0（6）位的特异性甲基化以及0（6） - 甲基鸟嘌呤与7 - 甲基鸟嘌呤的比例显著增加。未成年小鼠肾脏中的甲基化减少，而成年小鼠则增加。在肺中，未成年和成年小鼠蛋氨酸 - 半胱氨酸缺乏后，7 - 甲基鸟嘌呤的量均显著升高。结果表明，蛋氨酸 - 半胱氨酸缺乏饮食使肝脏中鸟嘌呤在0（6）位的特异性甲基化增加，同时两个年龄组器官之间的甲基化模式存在差异。
