Abushahba Faleh, Stiller Adrian, Mohamad Sherif A, Areid Nagat, Hupa Leena, Heino Terhi J, Vallittu Pekka K, Närhi Timo O
Department of Biomaterials Science and Turku Clinical Biomaterials Center-TCBC, Institute of Dentistry, University of Turku, Turku, Finland.
Department of Prosthetic Dentistry and Stomatognathic Physiology, Institute of Dentistry, University of Turku, Turku, Finland.
J Biomed Mater Res A. 2025 Jun;113(6):e37949. doi: 10.1002/jbm.a.37949.
This study investigated the chemical events that occur when titanium (Ti) surfaces are treated with air particle abrasion (APA) using zinc-containing bioactive glass (ZnBG), followed by immersion in simulated body fluid (SBF) for up to 96 h. The impact of these changes on osteoblast cell viability, adhesion, and differentiation was evaluated. Sandblasted and acid-etched (SA) Ti disks were subjected to APA with ZnBG particles and then immersed in SBF from 8 to 96 h. Ion dissolution and characterization of ZnBG powder and Ti disks were conducted. Analyses of osteoblast viability, adhesion, and alkaline phosphatase (ALP) activity were performed on MC3T3-E1 cells cultured on control disks (SA-Ti), as well as on ZnBG abraded disks (APA-Ti) and disks immersed for 96 h in SBF (CaP-Ti). After SBF immersion, the ZnBG particle surfaces showed a rise in Si atomic (at.)% within the first 8 h, while Ca remained stable, and the P doubled over 96 h. The ZnBG covering the disks dissolved during the first 8 h, and then the Ca, P, and Si at.% increased as the immersion time extended. The glass particles exhibited amorphous calcium-phosphate (Ca-P) layer formation after 96 h. A significantly (p = 0.004) higher cell viability level was observed on day 7 on APA-Ti compared to SA-Ti disks, while no differences in osteoblast differentiation were observed across the different surfaces. Fluorescence images demonstrated that on day 3, cells adhered to valleys and peaks of CaP-Ti threads but only to valleys on SA-Ti and APA-Ti disks. By day 7, cells were also observed on APA-Ti peaks but not on SA-Ti. In summary, APA enhanced osteoblast proliferation, and a biocompatible Ca-P layer, which formed upon mineralization, supported osteoblast viability, adhesion, and spreading.
本研究调查了使用含锌生物活性玻璃（ZnBG）对钛（Ti）表面进行空气颗粒磨损（APA）处理，随后在模拟体液（SBF）中浸泡长达96小时时发生的化学变化。评估了这些变化对成骨细胞活力、黏附及分化的影响。对喷砂和酸蚀（SA）钛盘用ZnBG颗粒进行APA处理，然后在SBF中浸泡8至96小时。对ZnBG粉末和钛盘进行离子溶解及表征。对在对照盘（SA-Ti）、ZnBG磨损盘（APA-Ti）以及在SBF中浸泡96小时的盘（CaP-Ti）上培养的MC3T3-E1细胞进行成骨细胞活力、黏附及碱性磷酸酶（ALP）活性分析。在SBF浸泡后，ZnBG颗粒表面在最初8小时内硅原子（at.）%上升，而钙保持稳定，磷在96小时内翻倍。覆盖在盘上的ZnBG在最初8小时内溶解，然后随着浸泡时间延长，钙、磷和硅的at.%增加。96小时后玻璃颗粒呈现无定形磷酸钙（Ca-P）层形成。与SA-Ti盘相比，在第7天观察到APA-Ti上的细胞活力水平显著更高（p = 0.004），而在不同表面之间未观察到成骨细胞分化的差异。荧光图像显示，在第3天，细胞黏附于CaP-Ti螺纹的谷和峰，但仅黏附于SA-Ti和APA-Ti盘的谷。到第7天，在APA-Ti的峰上也观察到细胞，但在SA-Ti上未观察到。总之，APA增强了成骨细胞增殖，矿化形成的生物相容性Ca-P层支持成骨细胞活力、黏附及铺展。
