Swanson J C, Suttie J W
Biochemistry. 1985 Jul 16;24(15):3890-7. doi: 10.1021/bi00336a012.
Plasma and hepatic microsomal forms of rat prothrombin have been compared by sodium dodecyl sulfate-polyacrylamide electrophoresis and isoelectric focusing. The major prothrombin species that accumulated in the microsomes of rats treated with warfarin had a molecular weight of 78 500 and a pI in 8 M urea of 6.3-6.5. Plasma prothrombin had a molecular weight of 83 500 and a pI of 5.3-5.7. Microsomes from normal rat liver contain a second pool of precursor with a molecular weight of 83 500, and digestion with the glycosidase Endo H indicated that this form has been processed to contain complex carbohydrates, while the Mr 78 500 form is a high mannose form and is the substrate for the vitamin K dependent carboxylase. Treatment of rats with tunicamycin revealed that glycosylation was not essential for carboxylation or secretion from the liver. Comparison of the aglyco forms of prothrombin and its precursors suggests that the intracellular forms contain a basic, Mr approximately 1500 peptide that is missing from the plasma form of prothrombin.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和等电聚焦法，对大鼠凝血酶原的血浆和肝微粒体形式进行了比较。在用华法林治疗的大鼠微粒体中积累的主要凝血酶原种类，其分子量为78500，在8M尿素中的等电点为6.3 - 6.5。血浆凝血酶原的分子量为83500，等电点为5.3 - 5.7。正常大鼠肝脏的微粒体含有分子量为83500的第二种前体池，用糖苷酶内切糖苷酶H消化表明，这种形式已被加工成含有复合碳水化合物，而分子量为78500的形式是高甘露糖形式，是维生素K依赖性羧化酶的底物。用衣霉素处理大鼠表明，糖基化对于羧化或从肝脏分泌不是必需的。凝血酶原及其前体的无糖基形式的比较表明，细胞内形式含有一种碱性的、分子量约为1500的肽，而凝血酶原的血浆形式中没有这种肽。
