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Biochim Biophys Acta. 1985 Oct 30;847(1):147-54. doi: 10.1016/0167-4889(85)90167-3.
This study tested the relationship between intact cell ATP levels and glucocorticoid receptor-binding capacity in the AtT-20 mouse pituitary tumor cell. Four treatments were used to alter intact cell ATP levels: incubation with NaF, incubation with 2,4-dinitrophenol, alterations in culture age, and incubation with dexamethasone. Each of these treatments clearly altered glucocorticoid receptor-binding capacity. Their effects on ATP levels, however, were not the same: NaF decreased ATP levels, dinitrophenol decreased ATP levels only transiently, and the last two treatments (increasing culture age and incubation with dexamethasone) produced an inverse relationship between ATP levels and receptor-binding capacity. It is concluded that, at least in the intact AtT-20 cell, receptor-binding capacity is not always directly related to ATP levels.
本研究检测了AtT - 20小鼠垂体瘤细胞中完整细胞ATP水平与糖皮质激素受体结合能力之间的关系。采用四种处理方法来改变完整细胞的ATP水平：用氟化钠孵育、用2,4 -二硝基苯酚孵育、改变培养时间以及用地塞米松孵育。这些处理方法均明显改变了糖皮质激素受体结合能力。然而，它们对ATP水平的影响并不相同：氟化钠降低了ATP水平，二硝基苯酚只是短暂地降低了ATP水平，而最后两种处理方法（延长培养时间和用地塞米松孵育）使ATP水平与受体结合能力之间呈现出负相关关系。得出的结论是，至少在完整的AtT - 20细胞中，受体结合能力并不总是与ATP水平直接相关。
