Berta Dereje Mengesha, Chane Elias, Tarekegn Amare Mekuanint, Cherie Negesse, Mulatie Zewudu, Kelem Amanuel, Addisu Bedasa, Teketelew Bisrat Brike
Department of Hematology and Immunohematology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences University of Gondar Gondar Ethiopia.
Department of Clinical Chemistry, School of Biomedical and Laboratory Science, College of Medicine and Health Sciences University of Gondar Gondar Ethiopia.
Health Sci Rep. 2025 Jun 16;8(6):e70925. doi: 10.1002/hsr2.70925. eCollection 2025 Jun.
The rejection of the specimens can cause delays in diagnosis, putting patients in repeated specimen collection, leading to inconvenience and prolonged turnaround time. As a result, the laboratories should periodically determine the frequency of specimen rejections. The aim of this study is to assess the hematology laboratory specimen rejection rate and its associated factors.
The cross-sectional study was conducted from September 2022 to February 2023. To assess the sample rejection rate and its associated factors, all blood specimens delivered to the laboratory during the study period were included. Data used to assess the sample rejection rate were collected using checklists, while data used to assess associated factors were collected through interviews. Epidata and STATA software were used for data enter and analysis, respectively. Descriptive statistics, along with bivariate and multivariate logistic analyses were used. The strength of the association between dependent and independent variables was measured using a 95% confidence interval.
A total of 27,242 blood specimens were assessed. From these, 982/27,242 (3.6%) blood specimens were rejected. Most of the specimens rejected were those submitted for manual hematocrit 190/1110 (17.1%) and peripheral morphology 30/170 (17.64%). The primary reasons for specimen rejection were hemolysis 239/27,242 (0.88%) and low-specimen quantity 221/27,242 (0.8%) were the main reasons for specimen rejection. Lack of adherence to standard operation procedures (SOP), specimens from the inpatient department (IPD). specimens from the emergency department, and specimens collected by non-laboratory health professionals increased the likelihood of sample rejection rate by 6.67, 2.44, 2.23, and 3.54 times, respectively.
The study found a relatively high specimen rejection rate in the study area. Thus, concerned bodies should provide need-based training for all professionals involved in hematology laboratory sample processing by prioritizing the most common causes and aggravating factors.
标本拒收会导致诊断延迟，使患者反复采集标本，带来不便并延长周转时间。因此，实验室应定期确定标本拒收的频率。本研究的目的是评估血液学实验室标本拒收率及其相关因素。
本横断面研究于2022年9月至2023年2月进行。为评估样本拒收率及其相关因素，纳入了研究期间送至实验室的所有血液标本。用于评估样本拒收率的数据通过检查表收集，而用于评估相关因素的数据通过访谈收集。分别使用Epidata和STATA软件进行数据录入和分析。采用描述性统计以及双变量和多变量逻辑分析。使用95%置信区间测量自变量和因变量之间的关联强度。
共评估了27242份血液标本。其中，982/27242（3.6%）份血液标本被拒收。大多数被拒收的标本是用于手工红细胞压积检测的190/1110（17.1%）和外周血形态学检测的30/170（17.64%）。标本拒收的主要原因是溶血239/27242（0.88%）和标本量不足221/27242（0.8%）。未遵守标准操作程序（SOP）、来自住院部（IPD）的标本、来自急诊科的标本以及由非实验室卫生专业人员采集的标本使样本拒收率增加的可能性分别为6.67倍、2.44倍、2.23倍和3.54倍。
研究发现研究区域标本拒收率相对较高。因此，相关机构应针对血液学实验室样本处理所涉及的所有专业人员，根据需求进行培训，优先关注最常见的原因和加重因素。
