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实验室工作人员培训研讨会对凝血样本拒收率的影响。

The impact of laboratory staff training workshops on coagulation specimen rejection rates.

作者信息

du Toit Marcel, Chapanduka Zivanai C, Zemlin Annalise E

机构信息

Division of Haematological Pathology, Department of Pathology, National Health Laboratory Service, Tygerberg Academic Hospital, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Division of Chemical Pathology, Department of Pathology, National Health Laboratory Service, Tygerberg Academic Hospital, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

出版信息

PLoS One. 2022 Jun 3;17(6):e0268764. doi: 10.1371/journal.pone.0268764. eCollection 2022.

Abstract

BACKGROUND

Pre-analytical variables can have a significant adverse impact on the quality and credibility of coagulation test results. Therefore, correct and consistent identification of pre-analytical variables that compromise coagulation specimen quality is of paramount importance. Lack of standardization and heterogeneity among laboratory staff when assessing coagulation specimens can lead to inconsistent identification of these variables. Failure to recognize such pre-analytical variables results in the analysis of poor quality specimens and the authorization of spurious test results.

OBJECTIVES

To determine the impact of a laboratory staff training workshop on coagulation specimen rejection rates and to ascertain the level of knowledge of laboratory personnel concerning coagulation specimen rejection criteria before and after the workshop.

METHODS

A retrospective three-month audit was performed with rejection data of incorrect blood to additive ratio, clotted, aged and haemolysed specimens collected. Training workshops and evaluation sessions were subsequently presented. A revised standard operating procedure delineating coagulation specimen rejection criteria was implemented and a repeat three-month audit was conducted.

RESULTS

In total, 13 162 coagulation specimens were received during the initial audit with 1 104 specimens (8.39%) rejected. Following the workshops, the rejection rate increased by 3.49% to 11.88% with 12 743 coagulation specimens received and 1 514 specimens rejected. Evaluation sessions performed before and after the workshops revealed that 95.2% of attendees attained improved knowledge.

CONCLUSION

This study demonstrated the pivotal importance of regular laboratory staff training. The increase in specimen rejection following the workshops signifies their success in educating laboratory personnel regarding the correct identification of pre-analytical variables. Since most pre-analytical variables occur outside the laboratory, educational workshops need to be extended to non-laboratory personnel responsible for specimen collection and transport.

摘要

背景

分析前变量可能对凝血检测结果的质量和可信度产生重大不利影响。因此,正确且一致地识别影响凝血标本质量的分析前变量至关重要。实验室工作人员在评估凝血标本时缺乏标准化和存在异质性,可能导致对这些变量的识别不一致。未能识别此类分析前变量会导致对质量不佳的标本进行分析,并认可虚假的检测结果。

目的

确定实验室工作人员培训研讨会对凝血标本拒收率的影响,并确定研讨会前后实验室人员对凝血标本拒收标准的知晓程度。

方法

对收集的不正确血液与添加剂比例、凝血、陈旧和溶血标本的拒收数据进行了为期三个月的回顾性审核。随后举办了培训研讨会和评估会议。实施了一份修订后的标准操作程序,该程序规定了凝血标本的拒收标准,并进行了为期三个月的重复审核。

结果

在初次审核期间,共收到13162份凝血标本,其中1104份标本(8.39%)被拒收。研讨会之后,拒收率上升了3.49%,达到11.88%,共收到12743份凝血标本,1514份标本被拒收。在研讨会前后进行的评估会议显示,95.2%的与会者知识水平有所提高。

结论

本研究证明了实验室工作人员定期培训的关键重要性。研讨会后标本拒收率的增加表明培训在教育实验室人员正确识别分析前变量方面取得了成功。由于大多数分析前变量发生在实验室之外,因此需要将教育研讨会扩展到负责标本采集和运输的非实验室人员。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e8/9165799/8665a9a9574c/pone.0268764.g001.jpg

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