Penot-Raquin Mathias, Sivia Mandeep, Fafoumi Kelly M, Larson Raegan, Dorrell Richard G, Dacks Joel B
Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Université Paris, 75005 Paris, France.
Research Federation for the study of Global Ocean Systems Ecology and Evolution, FR2022/Tara Oceans, 75016 Paris, France.
iScience. 2025 May 15;28(6):112679. doi: 10.1016/j.isci.2025.112679. eCollection 2025 Jun 20.
Classical cell biology paradigms are largely established on animal, fungal, and plant models which constitute a small fraction of eukaryotic diversity. Some important cellular machinery has been historically overlooked due to their absence from animals and fungi, e.g., the membrane-trafficking complex TSET involved in plant cell division and endocytosis. Here, we document TSET complexes in distantly related photosynthetic eukaryotic groups (green algae, red algae, haptophytes, cryptophytes, and stramenopiles including diatoms). 3D modeling predicts that at least some stramenopile-encoded subunits share conserved structural features with plant orthologues, and gene expression analysis from the diatom shows that they are co-expressed with endomembrane trafficking proteins. Finally, diatom TSET genes are detectable in -transcriptomic data from Oceans, suggesting functional roles in the wild. These results support the importance of integrating non-model organisms into our understanding of eukaryotic cell biology, as they may reveal underappreciated protein complexes essential for cellular and ecosystem functions.
经典细胞生物学范式主要建立在动物、真菌和植物模型之上，而这些模型只占真核生物多样性的一小部分。一些重要的细胞机制由于在动物和真菌中不存在，在历史上一直被忽视，例如参与植物细胞分裂和内吞作用的膜运输复合体TSET。在这里，我们记录了远缘光合真核生物类群（绿藻、红藻、定鞭藻、隐藻和包括硅藻在内的不等鞭毛藻）中的TSET复合体。三维建模预测，至少一些不等鞭毛藻编码的亚基与植物直系同源物具有保守的结构特征，硅藻的基因表达分析表明它们与内膜运输蛋白共表达。最后，在来自海洋的转录组数据中可检测到硅藻TSET基因，这表明它们在自然环境中具有功能作用。这些结果支持了将非模式生物纳入我们对真核细胞生物学理解的重要性，因为它们可能揭示对细胞和生态系统功能至关重要但未得到充分认识的蛋白质复合体。
