Liu Xiaohan, Fu Shijiao, Zhao Jialong, Gu Chenjie, Yang Danting, Jiang Tao
School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, P. R. China.
Analyst. 2025 Jul 7;150(14):3177-3187. doi: 10.1039/d5an00454c.
Foodborne pathogenic bacteria, acting as direct or indirect contaminants of food and water sources, have emerged as a significant global food safety issue. Thus, the advancement of rapid, sensitive, and precise methods for detecting foodborne pathogens is of paramount importance for effective food safety monitoring. In response to this critical need, we have successfully prepared a flexible PMMA-BP@MoS-based surface-enhanced Raman scattering (SERS) substrate through a two-step strategy involving hydrothermal synthesis and spin coating. This novel substrate enables label-free and highly sensitive detection of foodborne pathogens. The synergistic chemical enhancement effect of BP@MoS on target molecules, coupled with the highly efficient molecular enrichment capability endowed by PMMA integration, endows the proposed binary substrate with remarkable SERS enhancement properties and stability. These characteristics significantly reduce the detection limit of model molecules to as low as 6.67 × 10 M. Furthermore, trace levels of in raw meat samples were effectively detected using the PMMA-BP@MoS flexible substrate, achieving a wide linear detection range from 6.46 × 10 to 6.46 × 10 CFU mL. The flexible PMMA-BP@MoS SERS substrate emerged as a promising solution for nondestructive and highly sensitive bacterial detection, paving the way for clinical diagnostics of diseases caused by foodborne pathogens and offering a valuable tool to address food safety challenges.
食源性病原体作为食品和水源的直接或间接污染物，已成为一个重大的全球食品安全问题。因此，开发快速、灵敏且精确的食源性病原体检测方法对于有效的食品安全监测至关重要。为满足这一迫切需求，我们通过水热合成和旋涂两步法成功制备了一种基于聚甲基丙烯酸甲酯-黑磷@二硫化钼（PMMA-BP@MoS）的柔性表面增强拉曼散射（SERS）基底。这种新型基底能够实现对食源性病原体的无标记且高灵敏度检测。BP@MoS对目标分子的协同化学增强作用，加上PMMA整合赋予的高效分子富集能力，使所提出的二元基底具有显著的SERS增强特性和稳定性。这些特性将模型分子的检测限显著降低至低至6.67×10⁻⁸ M。此外，使用PMMA-BP@MoS柔性基底有效地检测了生肉样品中的痕量[具体物质未明确]，实现了从6.46×10²到6.46×10⁷ CFU/mL的宽线性检测范围。柔性PMMA-BP@MoS SERS基底成为一种有前途的无损且高灵敏度细菌检测解决方案，为食源性病原体引起的疾病的临床诊断铺平了道路，并为应对食品安全挑战提供了有价值的工具。
