呼吸道模拟培养基对抗真菌药敏试验的影响。
The impact of respiratory tract mimicking media on antifungal susceptibility testing.
作者信息
Vahedi-Shahandashti Roya, Hofer Isabella, Leighton Hollie, Fothergill Joanne L, Neill Daniel R, Lass-Flörl Cornelia
机构信息
Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, , Liverpool, United Kingdom.
出版信息
Antimicrob Agents Chemother. 2025 Aug 6;69(8):e0022525. doi: 10.1128/aac.00225-25. Epub 2025 Jun 17.
Abstract
The rise in antifungal resistance and changing epidemiology underscores the need to improve antifungal susceptibility testing. This study tested the standard medium RPMI and healthy lung and sinus media against , , and species. showed greater variability in minimum inhibitory concentrations (MICs) compared with molds, with higher MICs in respiratory tract-mimicking media than in RPMI. was the most affected mold, showing higher MICs for certain antifungals in these media.
摘要
抗真菌耐药性的上升和流行病学的变化凸显了改进抗真菌药敏试验的必要性。本研究针对[具体菌种1]、[具体菌种2]和[具体菌种3]，对标准培养基RPMI以及健康肺组织和鼻窦组织培养基进行了测试。与霉菌相比，[具体菌种1]在最低抑菌浓度（MICs）方面表现出更大的变异性，在模拟呼吸道的培养基中的MICs高于在RPMI中的。[具体菌种2]是受影响最大的霉菌，在这些培养基中对某些抗真菌药物表现出较高的MICs。
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J Fungi (Basel). 2023 Sep 26;9(10):966. doi: 10.3390/jof9100966.
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