Wang Dun-Ju, Ding Ming-Zhu, Hou Zheng-Jie, Zhang Yong, Shang Wei, Duan Tian-Xu, Xu Qiu-Man, Cheng Jing-Sheng
State Key Laboratory of Synthetic Biology, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, P. R. China.
Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, P. R. China.
ACS Synth Biol. 2025 Jul 18;14(7):2644-2656. doi: 10.1021/acssynbio.5c00101. Epub 2025 Jun 17.
As a lipopeptide, fengycin exhibits environmentally friendly, safe, and long-lasting biocontrol efficacy. However, due to its complex structure and the challenges in chemical synthesis, it is primarily produced through biosynthesis. This study reports an improvement in fengycin production in by engineering the central carbon metabolic pathway and blocking the carbon overflow pathway. The highest production achieved 1290.31 mg/L, representing a 2.05-fold increase compared to the original strain. Additionally, a coculture system was established in which supplied proline to strain CGF-PA, achieving a further increase in production to 2491.97 mg/L. The fengycin homologues were characterized using IMS-MS and separated by preparative liquid chromatography. The antifungal activities of fengycin homologues were quantitatively evaluated against , , , and , and their morphological changes were observed. The study also investigated the differences in antifungal activity among the fengycin variants. Components 4, 5, 6, and 7 exhibited relatively strong antifungal activity, and the various components of fengycin were found to work synergistically.
作为一种脂肽，丰原素具有环境友好、安全且持久的生物防治功效。然而，由于其结构复杂以及化学合成方面的挑战，它主要通过生物合成来生产。本研究报告了通过改造中心碳代谢途径和阻断碳溢流途径来提高丰原素产量的方法。最高产量达到1290.31毫克/升，与原始菌株相比提高了2.05倍。此外，还建立了一种共培养系统，其中 为菌株CGF-PA提供脯氨酸，使产量进一步提高到2491.97毫克/升。使用离子淌度质谱（IMS-MS）对丰原素同系物进行了表征，并通过制备液相色谱进行了分离。对丰原素同系物对 、 、 和 的抗真菌活性进行了定量评估，并观察了它们的形态变化。该研究还调查了丰原素变体之间抗真菌活性的差异。组分4、5、6和7表现出相对较强的抗真菌活性，并且发现丰原素的各种组分具有协同作用。
