Itani Z S
Dermatologica. 1977;154(5):273-6.
An easy and cheap method for culturing Neisseria is developed. The medium for gonococci is prepared as proposed by the producer (BBL, Oxoid, Hoechst). About 8 ml of the medium are poured in sterile air-tight stool tubes of 25 ml volume. Materials to be examined for gonococci are taken from the cervix, ureter or anus and are inoculated on the medium. A small piece (20-30 mg) of the GasPak tablet (BBL) is then deposited in the tube and closed immediately. The GasPak tablet consists of sodium bicarbonate and citric acid which, if they come in contact with humidity, produce CO2 gas. The inoculated tube is then put in an incubator at 37 degrees C for 14-24 h. This method gives a good microbiological result. With the aid of the oxidase reaction the colonies take a brown-black color. For further differentiation of the species the sugar fermentation method is necessary.
一种培养奈瑟菌的简便廉价方法被开发出来。淋球菌培养基按照生产商（BBL、Oxoid、赫斯特）的建议制备。将约8毫升培养基倒入25毫升体积的无菌气密粪便管中。用于检测淋球菌的材料取自子宫颈、输尿管或肛门，并接种在培养基上。然后将一小片（20 - 30毫克）气体发生片（BBL）放入管中并立即封闭。气体发生片由碳酸氢钠和柠檬酸组成，它们与湿度接触时会产生二氧化碳气体。然后将接种后的管子放入37摄氏度的培养箱中培养14 - 24小时。该方法能得到良好的微生物学结果。借助氧化酶反应，菌落呈现棕黑色。为了进一步区分菌种，糖发酵方法是必要的。
