Blanchard Adam M, Leigh James A
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
Microb Genom. 2025 Jun;11(6). doi: 10.1099/mgen.0.001425.
is a leading cause of bovine mastitis, a disease of significant economic impact to the dairy industry. Understanding the genetic factors essential for bacterial survival in milk and during intramammary infection is crucial for developing effective interventions and reducing the reliance on antimicrobials. Some studies have looked at how certain genes affect the growth and spread of in milk, but they have mostly ignored the impact of inflammation and increased blood vessel permeability.Cultures in milk containing serum subsequently exhibited accelerated growth rate and yield in proportion to the concentration of serum, potentially highlighting a role in bacterial proliferation. Using high-throughput transposon insertion sequencing, genes essential for to grow in milk and milk supplemented with serum, mimicking conditions of an inflamed mammary gland, were identified. A total of 359 genes associated with growth in milk and 460 genes required in milk supplemented with serum were identified and correlated with metabolic pathways crucial for the survival of in these phenotypes. Individual genes associated with carbohydrate metabolism, lipid metabolism and protein synthesis were significantly enriched in both conditions; however, there were a high number of genes with an unknown function, highlighting the poor characterization of the genome. There were also numerous hypothetical genes which hint at a putative role in bacterial proliferation, presenting opportunities for further research.
是引起牛乳腺炎的主要原因，这种疾病对乳制品行业具有重大经济影响。了解细菌在牛奶中以及乳腺内感染期间生存所必需的遗传因素，对于制定有效的干预措施和减少对抗生素的依赖至关重要。一些研究探讨了某些基因如何影响细菌在牛奶中的生长和传播，但大多忽略了炎症和血管通透性增加的影响。随后，含有血清的牛奶培养物显示出与血清浓度成比例的加速生长速率和产量，这可能突出了血清在细菌增殖中的作用。利用高通量转座子插入测序技术，确定了细菌在牛奶以及模拟炎症乳腺条件的添加血清的牛奶中生长所必需的基因。共鉴定出359个与在牛奶中生长相关的基因以及460个在添加血清的牛奶中生长所需的基因，并将它们与这些表型中细菌生存至关重要的代谢途径相关联。在这两种情况下，与碳水化合物代谢、脂质代谢和蛋白质合成相关的单个基因均显著富集；然而，存在大量功能未知的基因，这突出表明对该细菌基因组的表征不足。此外，还有许多假设基因暗示其在细菌增殖中可能发挥的作用，为进一步研究提供了机会。
