Durigon Daniele C, Marinho Rafaella B, Magnabosco Laura G, Piermartiri Tetsade C B, Göbel Lucas, Tasca Carla I, Xavier Fernando R, Nedel Cláudia B, Bortoluzzi Adailton J, Terenzi Hernán, Peralta Rosely A
Laboratório de Biologia Molecular Estrutural (LABIME), Departamento de Bioquímica, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, Santa Catarina, Brazil; Laboratório de Química Bioinorgânica e Cristalografia (LABINC), Departamento de Química, Universidade Federal de Santa Catarina, UFSC, 88040-900 Florianópolis, Santa Catarina, Brazil.
Laboratório de Química Bioinorgânica e Cristalografia (LABINC), Departamento de Química, Universidade Federal de Santa Catarina, UFSC, 88040-900 Florianópolis, Santa Catarina, Brazil.
J Inorg Biochem. 2025 Oct;271:112974. doi: 10.1016/j.jinorgbio.2025.112974. Epub 2025 Jun 10.
In this study, a novel ligand: 2-((bis(2-(phenylthio)ethyl)amino)methyl)-6-((bis(pyridin-2-ylmethyl)amino)methyl)-4-methylphenol (HL) was prepared and subsequently used to synthesize the complexes [Zn(L)(OAc)]ClO (1) and Fe(L)(μ-OAc)(μ-O) (2). The metal complexes were comprehensively characterized using different techniques such as IR, Mass Spectrometry, UV-Vis, elemental analysis and X-ray analysis. The crystal structures show that compound 1 is a mononuclear pentacoordinate zinc(II) complex, while compound 2 is a dinuclear hexacoordinate iron(III) compound, with the metal centers connected by μ-oxo and μ-acetato bridges. In both cases, the phenol-based ligand features thioether substituents as pendant arms. We have evaluated the binding of the new complexes to DNA and also their capacity to cleave it. Both complexes have excellent antitumor activity against an astrocytic tumor derived from a primary human GBM1 cell line. SYNOPSIS: The paper reports synthesis and characterization of two new complexes [Zn(L)(OAc)]ClO (1) and Fe(L)(μ-OAc)(μ-O) (2). Complex 1 exhibits better activity than 2 at lower concentrations and with a faster rate of action, showing a preference for the minor groove. The cytotoxicity studies demonstrated a reduction in cell viability when exposed to the complexes, with GBM and C6 cells.
在本研究中，制备了一种新型配体：2-((双(2-(苯硫基)乙基)氨基)甲基)-6-((双(吡啶-2-基甲基)氨基)甲基)-4-甲基苯酚(HL)，随后用于合成配合物[Zn(L)(OAc)]ClO (1)和Fe(L)(μ-OAc)(μ-O) (2)。使用红外光谱、质谱、紫外可见光谱、元素分析和X射线分析等不同技术对金属配合物进行了全面表征。晶体结构表明，化合物1是单核五配位锌(II)配合物，而化合物2是双核六配位铁(III)化合物，金属中心通过μ-氧和μ-乙酸根桥连接。在这两种情况下，基于苯酚的配体都具有硫醚取代基作为侧链。我们评估了新配合物与DNA的结合及其切割DNA的能力。两种配合物对源自原发性人GBM1细胞系的心形细胞瘤均具有优异的抗肿瘤活性。摘要：本文报道了两种新配合物[Zn(L)(OAc)]ClO (1)和Fe(L)(μ-OAc)(μ-O) (2)的合成与表征。配合物1在较低浓度下表现出比2更好的活性，作用速率更快，对小沟有偏好。细胞毒性研究表明，当GBM和C6细胞暴露于配合物时，细胞活力降低。
