Bullock G R, Leprán I, Parratt J R, Szekeres L, Wainwright C L
Br J Pharmacol. 1985 Sep;86(1):235-40. doi: 10.1111/j.1476-5381.1985.tb09454.x.
The effects of acute pretreatment with metoprolol, dazmegrel and a combination of these two drugs has been examined on myocardial infarct size in rats. Ischaemic damage was assessed 4 h after coronary artery occlusion in anaesthetized rats and after 48 h of ischaemia in conscious rats. Infarct size was measured histochemically (by using periodic-acid-Schiff diastase reaction for glycogen) and by standard histological examination (haematoxylin and eosin stain). There was some evidence of protection of the myocardium by metoprolol following 4 h of ischaemia (determined histologically) but this was not apparent 48 h after occlusion. When given alone, dazmegrel had no significant effects on infarct size assessed by either method. A clear reduction in the extent of glycogen depletion and histological damage was observed with the combination of metoprolol and dazmegrel 48 h after the onset of ischaemia. This protection was seen to occur in the horizontal plane of the heart, preventing the extension of the infarct towards the posterior wall of the left ventricle and showing some salvage of the epicardial surfaces.
已研究了美托洛尔、达唑米格列以及这两种药物联合进行急性预处理对大鼠心肌梗死面积的影响。在麻醉大鼠冠状动脉闭塞4小时后以及清醒大鼠缺血48小时后评估缺血损伤。通过组织化学方法（使用高碘酸 - 希夫淀粉酶反应检测糖原）和标准组织学检查（苏木精和伊红染色）测量梗死面积。缺血4小时后（通过组织学确定），有一些证据表明美托洛尔对心肌有保护作用，但在闭塞48小时后这种保护作用并不明显。单独使用时，达唑米格列对通过任何一种方法评估的梗死面积均无显著影响。缺血发作48小时后，美托洛尔和达唑米格列联合使用可明显减少糖原消耗程度和组织学损伤。这种保护作用见于心脏的水平面，可防止梗死向左心室后壁扩展，并显示出对心外膜表面的一些挽救作用。
