Johnson H G, McNee M L
Br J Pharmacol. 1985 Sep;86(1):63-7. doi: 10.1111/j.1476-5381.1985.tb09435.x.
Adenosine alone at 0.1 and 1.0 mg per tracheal segment stimulated mucus secretion by 52% and 88%, respectively, compared to baseline (P less than 0.0001). The site of the potent secretagogue effect of adenosine in canine trachea was consistent with A2 activation. A2 site activation and enhanced secretion were also induced by N-ethylcarboxamide adenosine and dipyridamole. N6-R-phenylisopropyl adenosine (PIA) and 2',5'-dideoxyadenosine (ddAdo) inhibited the adenosine-induced secretion (35% and 42%, respectively). However, when PIA or ddAdo were administered in conjunction with the potent phosphodiesterase inhibitor, methylisobutylxanthine (MIX), the effects of PIA were potentiated and the effects of ddAdo were reversed, yielding stimulation (A2) and antagonism (A1) of secretion, respectively. 8-Phenyltheophylline by aerosol was a very potent antagonist of the secretagogue effect of adenosine (70% inhibition; P less than 0.00001).
与基线相比，每气管节段单独给予0.1毫克和1.0毫克腺苷分别刺激黏液分泌增加52%和88%（P<0.0001）。腺苷在犬气管中产生有效促分泌作用的部位与A2激活一致。N-乙基甲酰胺腺苷和双嘧达莫也诱导了A2位点激活和分泌增强。N6-R-苯异丙基腺苷（PIA）和2',5'-二脱氧腺苷（ddAdo）抑制腺苷诱导的分泌（分别为35%和42%）。然而，当PIA或ddAdo与强效磷酸二酯酶抑制剂甲基异丁基黄嘌呤（MIX）联合给药时，PIA的作用增强，ddAdo的作用逆转，分别产生分泌刺激（A2）和拮抗（A1）作用。经气雾剂给予的8-苯基茶碱是腺苷促分泌作用的强效拮抗剂（抑制率70%；P<0.00001）。
