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通过乳化/内部凝胶化法将叶黄素包封于可得然胶和海藻酸钠中:微胶囊的构建、表征及稳定性

Lutein encapsulated in curdlan and sodium alginate by emulsification/internal gelation: Construction, characterization and stability of microcapsules.

作者信息

Wang Weichen, Qin Yanbo, Chen Ke, Gong Guangyi, Hou Zhanqun, Liu Libo, Li Chun, Zhang Guofang, Yan Huan, Hu Zhihua, He Jian

机构信息

Key Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Green Food Science Research Institute, National Dairy Engineering Technology Research Center, Harbin 150028, China.

Key Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, China.

出版信息

Int J Biol Macromol. 2025 Jul;318(Pt 4):145313. doi: 10.1016/j.ijbiomac.2025.145313. Epub 2025 Jun 15.

DOI:10.1016/j.ijbiomac.2025.145313
PMID:40527385
Abstract

In this study, microcapsules were constructed using the emulsification/internal ionic gelation method with curdlan (CUD) and sodium alginate (SA) as wall materials to enhance stability. Both one-way and response surface experiments were employed to optimize the microcapsule preparation process, resulting in an encapsulation rate of 81.36 %. XRD and DSC analyses indicated that CUD-SA composite microcapsules effectively encapsulate lutein (LUT) and enhance its stability. Rheological characterization demonstrated that the CUD-SA composite significantly enhanced gel strength, resulting in a more stable microcapsule structure. In addition, CUD-SA microcapsules exhibited significantly higher lutein loading (11.60 mg/g) compared to single-wall microcapsules. The stability assays demonstrated that CUD-SA-LUT microcapsules had higher retention under light, heat, and 4 °C storage conditions, as well as improved intestinal release rates. CUD-SA-LUT microcapsules demonstrated the lowest degradation constants and the longest half-life when compared to free LUT and single-wall microcapsules. These results demonstrated that the emulsification/internal gelation method is effective in enhancing the stability of lutein, thereby broadening their application in the food industry, and also serves as a reference for the application of CUD in the food sector.

摘要

在本研究中,以凝胶多糖(CUD)和海藻酸钠(SA)为壁材,采用乳化/内部离子凝胶法制备微胶囊以提高稳定性。采用单因素实验和响应面实验对微胶囊制备工艺进行优化,包封率达81.36%。X射线衍射(XRD)和差示扫描量热法(DSC)分析表明,CUD-SA复合微胶囊能有效包封叶黄素(LUT)并提高其稳定性。流变学表征表明,CUD-SA复合材料显著提高了凝胶强度,使微胶囊结构更稳定。此外,与单壁微胶囊相比,CUD-SA微胶囊的叶黄素载量显著更高(11.60 mg/g)。稳定性试验表明,CUD-SA-LUT微胶囊在光照、加热和4℃储存条件下具有更高的保留率,以及更高的肠道释放率。与游离LUT和单壁微胶囊相比,CUD-SA-LUT微胶囊的降解常数最低,半衰期最长。这些结果表明,乳化/内部凝胶法在提高叶黄素稳定性方面是有效的,从而拓宽了其在食品工业中的应用,也为CUD在食品领域的应用提供了参考。

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