Kendir Gülsen, Güleç Meltem, Öztürk Ayça Bal, Torkay Gülşah, Muhammed Muhammed Tilahun, Olgun Abdullah, Köroğlu Ayşegül
Department of Pharmaceutical Botany, Faculty of Pharmacy Süleyman Demirel University Isparta Türkiye.
Department of Pharmacognosy, Faculty of Pharmacy Istanbul University-Cerrahpasa Istanbul Türkiye.
Food Sci Nutr. 2025 Jun 17;13(6):e70399. doi: 10.1002/fsn3.70399. eCollection 2025 Jun.
(L.) Mill. has cultivated wild forms in many parts of the world, attracting notably with its fruit. Its fruits can be eaten raw or processed. Studies have shown that fruit juice has various pharmacological effects and significant nutritional value. This study investigated the phenolic composition of fruit juice. Caffeic acid was the major component. Cytotoxic activity and wound-healing effect were evaluated by in vitro assays. Results showed a concentration-dependent response, with the lowest viability (111.89% ± 17.90%) at 1000 g/mL and the highest (162.25% ± 7.48%) at 250 g/mL. The extract (0-250 g/mL) had comparable wound closure to the control group. It increased the lifespan of , an in vivo model, at 125 g/mL. The binding potential of to matrix metalloproteinase 13 (MMP-13) and glycogen synthase kinase 3 β (GSK3-β) was investigated for its wound-healing effect. The phytoconstituents are bound to MMP-13 and GSK3-β, but less than the native ligands. They bound more to MMP-13 than to GSK3-β. Caffeic acid showed the highest binding potential with MMP-13 and one of the highest with GSK3-β. This study suggests that the wound-healing and anti-aging effects of fruit juice can be promising.
(L.) 米尔. 在世界许多地方种植了野生品种，其果实尤其具有吸引力。其果实可生食或加工。研究表明，果汁具有多种药理作用和显著的营养价值。本研究调查了果汁的酚类成分。咖啡酸是主要成分。通过体外试验评估了细胞毒性活性和伤口愈合效果。结果显示出浓度依赖性反应，在1000μg/mL时活力最低（111.89%±17.90%），在250μg/mL时最高（162.25%±7.48%）。提取物（0 - 250μg/mL）与对照组的伤口闭合情况相当。在125μg/mL时，它延长了一种体内模型的寿命。研究了其对基质金属蛋白酶13（MMP - 13）和糖原合酶激酶3β（GSK3 - β）的结合潜力以探讨其伤口愈合作用。植物成分与MMP - 13和GSK3 - β结合，但比天然配体少。它们与MMP - 13的结合多于与GSK3 - β的结合。咖啡酸与MMP - 13的结合潜力最高，与GSK3 - β的结合潜力是最高之一。本研究表明，该果汁的伤口愈合和抗衰老作用可能很有前景。
