Shifa Ul Haq H M, Ashfaq Ramla, Mehmood Azra, Shahid Warda, Azam Ghufran, Azam Maryam, Tasneem Saba, Akram Shehla Javed, Malik Kausar, Riazuddin Sheikh
National Centre of Excellence in Molecular Biology, 87-West Canal Bank Road, University of Punjab, Lahore, 53700, Pakistan.
Genome Editing Lab, Food Biotechnology Research Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratory Complex, Lahore, 54600, Pakistan.
Regen Ther. 2023 Jan 24;22:115-127. doi: 10.1016/j.reth.2023.01.001. eCollection 2023 Mar.
The therapeutic effectiveness of stem cells after transplantation is hampered by the hypoxic milieu of chronic wounds. Prior research has established antioxidant priming as a thorough plan to improve stem cell performance. The purpose of this study was to ascertain how caffeic acid (CA) priming affected the ability of human adipose-derived stem cells (hASCs) to function under hypoxic stress. In order to study the cytoprotective properties of CA, hASCs were primed with CA in CoCl hypoxic conditions. Microscopy was used to assess cell morphology, while XTT, Trypan Blue, X-gal, LDH, Live Dead, scratch wound healing, and ROS assays were used to analyze viability, senescence, cell death, proliferation, and reactive oxygen species prevalence in the cells. According to our findings, CA priming enhances hASCs' ability to survive and regenerate in a hypoxic microenvironment more effectively than untreated hASCs. Our in-vitro research suggested that pre-treatment with CA of hASCs could be a unique way to enhance their therapeutic efficacy and ability to survive in hypoxic microenvironments.
移植后干细胞的治疗效果受到慢性伤口缺氧环境的阻碍。先前的研究已将抗氧化预处理确立为改善干细胞性能的全面方案。本研究的目的是确定咖啡酸（CA）预处理如何影响人脂肪来源干细胞（hASC）在缺氧应激下的功能能力。为了研究CA的细胞保护特性，在氯化钴缺氧条件下用CA对hASC进行预处理。使用显微镜评估细胞形态，同时使用XTT、台盼蓝、X-gal、乳酸脱氢酶、活死细胞、划痕伤口愈合和活性氧检测来分析细胞中的活力、衰老、细胞死亡、增殖和活性氧水平。根据我们的研究结果，与未处理的hASC相比，CA预处理能更有效地提高hASC在缺氧微环境中的存活和再生能力。我们的体外研究表明，用CA预处理hASC可能是增强其治疗效果和在缺氧微环境中存活能力的独特方法。
