卵孵化对血细胞募集及幼虫对病原体易感性的影响。
Impact of egg incubation on hemocyte recruitment and susceptibility of larvae to pathogens.
作者信息
Carmo Paulo Henrique Fonseca, de Lima Patrícia Michelle Nagai, Silva Fabiana Alves, Ribeiro Jaqueline Lemes, Junqueira Juliana Campos, Garcia Maíra Terra
机构信息
Departamento de Biociências e Diagnóstico Bucal, Instituto de Ciência e Tecnologia, Universidade Estadual Paulista (UNESP), São José dos Campos, Brazil.
Department of Genetics, Microbiology and Immunology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), Botucatu, Brazil.
出版信息
Front Microbiol. 2025 Jun 3;16:1611104. doi: 10.3389/fmicb.2025.1611104. eCollection 2025.
INTRODUCTION
is widely used as an model in microbiological and toxicological studies due to its reproducibility, low cost, and ease of handling. However, maintaining homogeneous laboratory colonies presents challenges. This study evaluated whether different incubation methods influence the larval immune response against , and .
METHODS
Four experimental groups were established: (1) Traditional group (TG), eggs stored at 27°C; (2) Immediate group (IG), eggs stored at 16°C for 120 days before incubation at 27°C; (3) Gradual group (GG), temperature gradually reduced from 27°C to 16°C over 98 days, then increased back; and (4) Frozen group (FG), eggs stored at freezing temperatures.
RESULTS AND DISCUSSION
FG eggs were non-viable, and IG and GG showed delayed development. IG larvae were more susceptible to infections, while GG larvae resembled TG. Thus, GG can serve as an effective alternative rearing method.
引言
由于其可重复性、低成本和易于操作，在微生物学和毒理学研究中被广泛用作模型。然而，维持实验室菌落的同质性存在挑战。本研究评估了不同的孵化方法是否会影响幼虫对[具体病原体1]和[具体病原体2]的免疫反应。
方法
设立四个实验组：（1）传统组（TG），卵在27°C下储存；（2）即时组（IG），卵在16°C下储存120天，然后在27°C下孵化；（3）渐变组（GG），温度在98天内从27°C逐渐降至16°C，然后再回升；（4）冷冻组（FG），卵在冷冻温度下储存。
结果与讨论
FG组的卵无法存活，IG组和GG组显示发育延迟。IG组的幼虫更容易受到感染，而GG组的幼虫与TG组相似。因此，GG组可作为一种有效的替代饲养方法。
Zotero 插件