Chakrabarty S, Jan Y, Miller C A, Brattain M G
Cancer Lett. 1985 Sep 30;28(3):291-7. doi: 10.1016/0304-3835(85)90037-0.
The nuclear protein and phosphoprotein profiles from 3 subpopulations of human colonic carcinoma cells which expressed different levels of neoplastic properties were characterized by two-dimensional electrophoresis. The silver stained nuclear protein profiles were found to be remarkably similar among the subpopulations. However, 2 types of nuclear proteins were found to be selectively modified by phosphorylation/dephosphorylation reactions. The dephosphorylation of type I and the phosphorylation of type II nuclear proteins were found to be associated with the HCT 116a subpopulation which expressed a high level of neoplastic properties. Conversely, the phosphorylation of type I and the dephosphorylation of type II nuclear proteins were found to be associated with the HCT 116b subpopulation, which expressed a low level of neoplastic properties. The HCT 116 subpopulation, which expressed an intermediate level of neoplastic properties, was found to possess an intermediate phosphoprotein profile relative to that of the other two subpopulations. Selective modification of cellular proteins by phosphorylation/dephosphorylation reactions may be involved in the generation of tumor cell diversity and heterogeneity.
通过二维电泳对表达不同水平肿瘤特性的3个人结肠癌细胞亚群的核蛋白和磷蛋白图谱进行了表征。发现银染的核蛋白图谱在这些亚群之间非常相似。然而，发现有2种核蛋白被磷酸化/去磷酸化反应选择性修饰。发现I型核蛋白的去磷酸化和II型核蛋白的磷酸化与表达高水平肿瘤特性的HCT 116a亚群相关。相反，发现I型核蛋白的磷酸化和II型核蛋白的去磷酸化与表达低水平肿瘤特性的HCT 116b亚群相关。表达中等水平肿瘤特性的HCT 116亚群相对于其他两个亚群具有中间磷蛋白图谱。磷酸化/去磷酸化反应对细胞蛋白的选择性修饰可能参与了肿瘤细胞多样性和异质性的产生。
