Senger D R, Perruzzi C A
Cancer Res. 1985 Nov;45(11 Pt 2):5818-23.
A wide variety of rodent tumor cells of both fibroblastic and epithelial origins secrete a major transformation-related phosphoprotein with a molecular weight of approximately 62,000. Tumorigenic cells, regardless of the transforming agent, secrete 10-fold or more of this 32P-labeled protein as compared with their nontumorigenic counterparts. In this study we have extended these previous findings to tumorigenic human cells of diverse origins (both sarcomas and carcinomas). Metabolic labeling of cells with [32P]orthophosphate and immunoprecipitation with antibody specifically directed against the rat transformation-dependent secreted phosphoprotein have been used to identify antigenically related human phosphoproteins (Mr 66,000-69,000). Of the 14 human cell lines examined, all 8 of the lethal tumorigenic cell populations secreted these phosphoproteins either in continuous culture or as fresh explants from nude mice while the six nonmalignant cell lines did not (tumorigenicity in all cases was assayed in nude mice). Included in our study were three tumorigenic human cell lines (two sarcomas, one carcinoma), each with a matched, nontumorigenic control. The very close correlation between secretion of these phosphoproteins and the tumor cell phenotype of both rodents and humans raises the possibility that they may be important for tumor growth in vivo.
多种源自成纤维细胞和上皮细胞的啮齿动物肿瘤细胞会分泌一种主要的与转化相关的磷蛋白，其分子量约为62,000。无论转化因子是什么，致瘤细胞分泌的这种经32P标记的蛋白比其非致瘤对应细胞多10倍或更多。在本研究中，我们将这些先前的发现扩展到了多种来源的致瘤人类细胞（肉瘤和癌）。用[32P]正磷酸盐对细胞进行代谢标记，并使用针对大鼠转化依赖性分泌磷蛋白的抗体进行免疫沉淀，以鉴定抗原相关的人类磷蛋白（分子量66,000 - 69,000）。在所检测的14个人类细胞系中，所有8个致死性致瘤细胞群体在连续培养中或作为来自裸鼠的新鲜外植体时都分泌这些磷蛋白，而6个非恶性细胞系则不分泌（所有情况下的致瘤性均在裸鼠中测定）。我们的研究包括三个致瘤人类细胞系（两个肉瘤，一个癌），每个都有一个匹配的非致瘤对照。这些磷蛋白的分泌与啮齿动物和人类肿瘤细胞表型之间的密切相关性增加了它们可能对体内肿瘤生长很重要的可能性。
