Reactive oxygen species (ROS), such as hydrogen peroxide (HO), are known signaling molecules that increase endothelial barrier permeability. In this study, we investigated the roles of redox-sensitive transient receptor potential (TRP) ion channels, TRPM2, TRPV2 and TRPV4, in HO-induced endothelial barrier dysfunction. Using primary human pulmonary microvascular endothelial cells (HPMEC), we employed impedance-based resistance measurements, Western blot, and immunofluorescence staining to assess the effects of HO on the endothelial barrier. Exposure to sublytic concentrations of HO caused an acute loss of endothelial barrier integrity, accompanied by the cleavage of vascular endothelial cadherin (VE-cadherin), which was also apparent after application of the TRPV2 activator cannabidiol. The inhibition of either TRPV2 with tranilast or a disintegrin and metalloprotease domain-containing protein 10 (ADAM10) with GI254023X significantly reduced HO-induced VE-cadherin cleavage, while TRPM2 inhibition by econazole significantly increased HO-driven VE-cadherin cleavage and blockage of TRPV4 showed no effect. Although inhibition of either TRPV2 or ADAM10 did not prevent the initial loss of barrier resistance upon HO exposure, both were essential for the subsequent recovery of barrier integrity. Time-course immunofluorescence stainings revealed that HPMEC barrier recovery involved a transient localization of N-cadherin proteins at adherens junctions. This process of cadherin-switching did not occur upon inhibition of TRPV2 or ADAM10. Our results highlight a novel role for TRPV2 as a redox sensitive ion channels in the microvascular endothelium and provide insight into the mechanisms underlying pulmonary microvascular endothelial barrier recovery.