Yang Jing, Zhu Danyang, Gong Hui, Cui Rongrong, Liu Xiaoguang, Wang Yayi, Makinia Jacek, Xie Li, Dai Xiaohu
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Key Laboratory of Urban Water Supply, Water Saving and Water Environment Governance in the Yangtze River Delta of Ministry of Water Resources, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2025 Oct;434:132831. doi: 10.1016/j.biortech.2025.132831. Epub 2025 Jun 16.
Practical applications of anaerobic ammonium oxidation (anammox) are often hindered by slow recovery following operational failures. In this study, a novel "0 + 1 > 1" phenomenon was observed when using mixed inactive-active sludge as inoculum. In this context, "0" represented inactive sludge caused by excessive aeration, while "1" denoted active sludge. The mixed inactive-active sludge achieved faster recovery than either inactive or active sludge alone under identical conditions, demonstrating "0 + 1 > 1". Two distinct anammox genera were used: Candidatus Kuenenia as active sludge while Candidatus Brocadia as inactive sludge, revealing the primary mechanism was enhanced growth of anammox bacteria from active sludge rather than the reactivation of inactive sludge, which was related with increased relative gene abundances of enzymes involved in N metabolism, enhanced amino acid synthesis and quorum sensing (QS) communication. Reactivation of inactive sludge was excluded due to irreversible damage of hdh. This study presents a cost-effective strategy for accelerating anammox process recovery.
厌氧氨氧化（anammox）的实际应用常常因运行故障后的缓慢恢复而受阻。在本研究中，当使用混合的非活性-活性污泥作为接种物时，观察到一种新型的“0 + 1 > 1”现象。在此背景下，“0”代表因曝气过度导致的非活性污泥，而“1”表示活性污泥。在相同条件下，混合的非活性-活性污泥比单独的非活性或活性污泥恢复得更快，证明了“0 + 1 > 1”。使用了两种不同的厌氧氨氧化菌属：“Candidatus Kuenenia”作为活性污泥，而“Candidatus Brocadia”作为非活性污泥，揭示其主要机制是活性污泥中厌氧氨氧化菌的生长增强，而非非活性污泥的重新激活，这与参与氮代谢的酶的相对基因丰度增加、氨基酸合成增强和群体感应（QS）通讯有关。由于hdh的不可逆损伤，排除了非活性污泥的重新激活。本研究提出了一种加速厌氧氨氧化过程恢复的经济有效策略。
