Ben-Shachar G, Arcilla R A, Lucas R V, Manasek F J
Circ Res. 1985 Nov;57(5):759-66. doi: 10.1161/01.res.57.5.759.
Sixty-two chick embryo hearts were studied at incremental stages of development (Hamburger-Hamilton stages 16 to 39) by scanning electron microscopy following 3% glutaraldehyde fixation and critical point drying. Early in cardiac development, the primitive ventricle becomes homogeneously trabeculated with highly organized sheets of myocytes lined by endocardial cells, with the trabeculae generally oriented in the dorsoventral direction. Coalescence of these trabecular sheets begins at stage 26, initially at the area of the bulboventricular flange, and later proceeding caudally toward the floor of the ventricle. The fusion process is finished by stage 30, resulting in a muscular ventricular septum that has now divided the primitive ventricle into right and left ventricles. Further growth of the ventricular septum is by continued fusion of the adjoining trabecular sheets. Remnants of the apposing trabecular sheets are found in the solidified muscular septum in the form of endocardial channels. We suggest that persistent patency of these channels results in muscular ventricular septal defects.
通过扫描电子显微镜，在3%戊二醛固定和临界点干燥后，对62个处于发育递增阶段（汉伯格-汉密尔顿第16至39阶段）的鸡胚心脏进行了研究。在心脏发育早期，原始心室均匀地形成小梁，由心肌细胞组成的高度有序的薄片被心内膜细胞覆盖，小梁通常沿背腹方向排列。这些小梁薄片的融合始于第26阶段，最初在球室嵴区域，随后向尾端朝着心室底部推进。融合过程在第30阶段完成，形成一个肌肉性室间隔，此时原始心室已被分为右心室和左心室。室间隔的进一步生长是通过相邻小梁薄片的持续融合实现的。在凝固的肌肉性间隔中，相对的小梁薄片的残余以心内膜通道的形式存在。我们认为这些通道的持续开放会导致肌肉性室间隔缺损。
