野生和体外培养的狭叶塞克罗皮亚树根部分的比较代谢谱分析揭示了五环三萜酸生物合成的保守性。
Comparative Metabolic Profiling of Wild and In Vitro Cultivated Cecropia angustifolia Roots Fraction Reveals Conservation of Pentacyclic Triterpene Acid Biosynthesis.
作者信息
Díaz-Sánchez Luis M, Chacón-Patiño Martha L, Blanco-Tirado Cristian, Combariza Marianny Y, Montoya Guillermo
机构信息
Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia.
Departamento de Química, Universidad de Pamplona, Pamplona, Norte de Santander, Colombia.
出版信息
Phytochem Anal. 2025 Aug;36(6):1829-1839. doi: 10.1002/pca.70001. Epub 2025 Jun 18.
INTRODUCTION
Cecropia angustifolia roots are a traditional source of pentacyclic triterpene acids (PTAs), bioactive compounds with promising therapeutic potential. In vitro cultivation offers a sustainable alternative to metabolite production while minimizing the impact on wild populations.
OBJECTIVE
To compare the metabolic profiles of wild and in vitro cultivated C. angustifolia roots using ultrahigh-resolution mass spectrometry.
METHODS
Root fractions from wild and in vitro cultivated materials were analyzed using negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-)-FT-ICR-MS) at 21 Tesla.
RESULTS
Molecular analysis revealed diverse PTA species in both wild and in vitro root fractions. While in vitro cultivation produced a more streamlined metabolic profile, PTA content remained significantly enriched.
CONCLUSION
In vitro cultivation of C. angustifolia roots represents a viable strategy for sustainably producing pharmacologically relevant PTAs, supporting both conservation and the biotechnological use of natural products.
引言
狭叶塞克罗皮亚树的根是五环三萜酸（PTAs）的传统来源，五环三萜酸是具有潜在治疗潜力的生物活性化合物。体外培养为代谢物生产提供了一种可持续的替代方法，同时将对野生种群的影响降至最低。
目的
使用超高分辨率质谱法比较野生和体外培养的狭叶塞克罗皮亚树根的代谢谱。
方法
使用21特斯拉的负离子电喷雾电离傅里叶变换离子回旋共振质谱（ESI(-)-FT-ICR-MS）分析野生和体外培养材料的根部分。
结果
分子分析揭示了野生和体外根部分中多种PTA种类。虽然体外培养产生了更简化的代谢谱，但PTA含量仍然显著富集。
结论
狭叶塞克罗皮亚树根的体外培养是可持续生产药理学相关PTA的可行策略，既支持保护又支持天然产物的生物技术应用。
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