Pocino M, Malavé I
Clin Immunol Immunopathol. 1985 Dec;37(3):324-33. doi: 10.1016/0090-1229(85)90102-3.
Cells binding peanut agglutinin (PNA) were studied in the thymus, spleen, and mesenteric lymph nodes from mice placed in the post weaning period on protein-restricted diets containing 8% (R8%) and 4% (R4%) casein. The proportion of PNA+ thymocytes and the absolute number of total and PNA+ cells in the thymus were significantly diminished in R8% and R4% mice. Larger proportion of PNA+ thymocytes showed weaker fluorescence in R8% and R4% than in normally fed (N) animals. Recovery of PNA+ thymocytes was observed in R8% but not in R4% mice at 8 weeks. The number of total and PNA+ cells was significantly diminished although the proportion of PNA+ cells was not modified in the peripheral lymphoid organs of R8% and R4% mice. Results indicate that protein restriction preferentially affected the immature cortical PNA+ cells in the thymus whereas cell depletion in the peripheral lymphoid organs occurred at the expense of both the PNA+ and PNA- subpopulations.
对处于断奶后时期、食用含8%（R8%）和4%（R4%）酪蛋白的蛋白质限制饮食的小鼠的胸腺、脾脏和肠系膜淋巴结中结合花生凝集素（PNA）的细胞进行了研究。在R8%和R4%饮食组小鼠中，胸腺中PNA+胸腺细胞的比例以及胸腺中总细胞和PNA+细胞的绝对数量均显著减少。与正常喂养（N）的动物相比，R8%和R4%饮食组中较大比例的PNA+胸腺细胞显示出较弱的荧光。在8周时，R8%饮食组小鼠中观察到PNA+胸腺细胞的恢复，但R4%饮食组小鼠未观察到。虽然R8%和R4%饮食组小鼠外周淋巴器官中PNA+细胞的比例未改变，但总细胞和PNA+细胞的数量显著减少。结果表明，蛋白质限制优先影响胸腺中未成熟的皮质PNA+细胞，而外周淋巴器官中的细胞减少是以PNA+和PNA-亚群为代价发生的。
