London J, Berrih S, Bach J F
J Immunol. 1978 Aug;121(2):438-43.
Fluorescein-coupled peanut agglutinin (PNA) has been used at the single-cell level to study mouse lymphocyte subpopulations. PNA not only binds to most thymocytes, as has already been shown by other authors, but also binds to a small fraction of peripheral lymphocytes that are all T cells (theta+Ig-) or null cells (theta-Ig-). Most PNA-positive thymocytes are sensitive to in vivo corticosteroids and irradiation (450 rads) treatments. Conversely, the positive spleen cells (5% of total spleen lymphocytes) are essentially resistant to corticosteroids and irradiation. Study of PNA binding during ontogenesis shows the occurrence of PNA-positive cells in the fetal liver before thymus constitution and in the very beginning of embryonic thymus and spleen development. These data indicate that PNA is a marker of early T cell subpopulations but that there are probably several distinct subsets of PNA-positive T cells.
荧光素偶联的花生凝集素（PNA）已被用于单细胞水平研究小鼠淋巴细胞亚群。正如其他作者已经表明的那样，PNA不仅能与大多数胸腺细胞结合，还能与一小部分外周淋巴细胞结合，这些外周淋巴细胞均为T细胞（θ+Ig-）或裸细胞（θ-Ig-）。大多数PNA阳性胸腺细胞对体内皮质类固醇和辐射（450拉德）治疗敏感。相反，阳性脾细胞（占脾淋巴细胞总数的5%）对皮质类固醇和辐射基本具有抗性。对个体发育过程中PNA结合的研究表明，在胸腺形成之前的胎肝以及胚胎胸腺和脾脏发育的最初阶段就出现了PNA阳性细胞。这些数据表明，PNA是早期T细胞亚群的标志物，但可能存在几个不同的PNA阳性T细胞亚群。
