Gad Hossam, Mahmoud Mohamed A, Antar Mohamed, Sayed Ahmed Ahmed, Laudanski Krzysztof
Department of Anesthesiology and Perioperative Care, Mayo Clinic, Rochester, MN, USA.
Division Pulmonary and Critical Care, Mayo Clinic, Rochester, MN, USA.
J Multidiscip Healthc. 2025 Jun 14;18:3447-3453. doi: 10.2147/JMDH.S515244. eCollection 2025.
Recent discoveries have pointed to the role of IGFALS immunological response to viral challenges, potentially leading to the emergence of a cytokine storm. Here, we investigate if serum IGFALS during the acute response to SARS-CoV-2 will not be likely to accompany immune response during acute phase and convalescence.
We recruited patients hospitalized with PCR-confirmed SARS-CoV-2 infection in 2020 and have blood collected after securing consent (t), at 48 hours (t), 7 days (t), and after discharge from hospital (t). IGFALS and IGF-1 in serum were measured to assess dynamics of the illness and compared against non-specific inflammatory (C-reactive protein, IL-6) markers. Serum titers of IgG against proteins S and N assessed specific viral responses. Serum HMGB-1 was measured to assess level of necrosis. Demographic and clinical data were collected using electronic health records (EHR). Survival was determined at six months from admission.
No difference between serum IGFALS and IGF-1 levels was seen across the studied time points. IGFALS showed significant positive correlations with IGFALS ( =0.18; <0.001), IGFALS (0.19; =0.004), and IGFALS ( 0.23; =0.045). IGFALS correlated negatively with IGF-1 but positively with growth hormone. IGFALS showed significant inverse correlations with serum levels of HMGB1 (0.26; <0.001) and t ( 0.27; <0.001). A significant correlation in the case of IGFALS and CRP ( 0.09, = 0.021), IGFALS and CRP ( 0.271, =0.039) was seen. Similar correlations were seen at 48 hours of sampling time for IL-6 ( 0.14, =0.006). In terms of specific antiviral response, we observed that serum IGFALS demonstrated correlation levels of serum IgG ( 0.09, =0.024). A positive correlation was found between length of stay in hospital or ICU and serum IGFALS
Though IGFALS serum levels did not change significantly during SARS-CoV-2 infection, we observed correlations with markers of tissue destruction, C-reactive protein, IL-6, and length of hospital stay.
最近的研究发现指出了胰岛素样生长因子结合蛋白酸不稳定亚基（IGFALS）在针对病毒挑战的免疫反应中的作用，这可能会引发细胞因子风暴。在此，我们研究在对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的急性反应期间，血清IGFALS是否不太可能在急性期和恢复期伴随免疫反应。
我们招募了2020年因PCR确诊为SARS-CoV-2感染而住院的患者，并在获得同意后采集血液（t0）、48小时后（t1）、7天后（t2）以及出院后（t3）。检测血清中的IGFALS和胰岛素样生长因子-1（IGF-1）以评估病情动态，并与非特异性炎症标志物（C反应蛋白、白细胞介素-6）进行比较。检测针对S蛋白和N蛋白的IgG血清滴度以评估特异性病毒反应。检测血清高迁移率族蛋白B1（HMGB-1）以评估坏死程度。使用电子健康记录（EHR）收集人口统计学和临床数据。从入院起六个月确定生存率。
在研究的各个时间点，血清IGFALS和IGF-1水平未见差异。IGFALS与IGFALS呈显著正相关（r = 0.18；P < 0.001）、IGFALS（r = 0.19；P = 0.004）以及IGFALS（r = 0.23；P = 0.045）。IGFALS与IGF-1呈负相关，但与生长激素呈正相关。IGFALS与血清HMGB1水平呈显著负相关（r = 0.26；P < 0.001）以及t（r = 0.27；P < 0.001）。IGFALS与C反应蛋白存在显著相关性（r = 0.09，P = 0.021）、IGFALS与C反应蛋白（r = 0.271，P = 0.039）。在采样时间48小时时，白细胞介素-6也有类似相关性（r = 0.14，P = 0.006）。在特异性抗病毒反应方面，我们观察到血清IGFALS与血清IgG的相关水平（r = 0.09，P = 0.024）。住院或入住重症监护病房（ICU）的时间长度与血清IGFALS之间存在正相关。
尽管在SARS-CoV-2感染期间IGFALS血清水平没有显著变化，但我们观察到其与组织破坏标志物、C反应蛋白、白细胞介素-6以及住院时间存在相关性。
