Ruíz Moleón Vera, Alende Charles, Fotouhi Maryam, Ayoubi Riham, Southern Kathleen, Laflamme Carl
Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
F1000Res. 2025 Jan 2;13:1049. doi: 10.12688/f1000research.155929.2. eCollection 2024.
Stimulator of interferon genes protein (STING1) is an immune adaptor protein which promotes innate immune defense mechanisms against pathogens. To enhance our understanding of STING1-associated disease, it is essential to make high-performing antibodies accessible to the scientific community. This study aims to improve reliability of STING1 research as we have characterized sixteen STING1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
干扰素基因刺激蛋白（STING1）是一种免疫衔接蛋白，可促进针对病原体的固有免疫防御机制。为了加深我们对STING1相关疾病的理解，使科学界能够获得高性能抗体至关重要。本研究旨在提高STING1研究的可靠性，因为我们使用了标准化实验方案，通过比较敲除细胞系和同基因亲本对照中的检测结果，对16种用于蛋白质免疫印迹、免疫沉淀和免疫荧光的STING1商业抗体进行了表征。这些研究是一项更大的合作计划的一部分，该计划旨在通过表征人源蛋白质的商业可用抗体并将结果公开作为科学界的资源来解决抗体可重复性问题。虽然不同实验室对抗体和实验方案的使用有所不同，但我们鼓励读者将本报告作为指南，为其特定需求选择最合适的抗体。
