高血小板分布宽度会影响通过无创产前检测所检测的游离DNA胎儿分数的检测。
High platelet distribution width affects the detection of fetal fraction of cell-free DNA detected by non-invasive prenatal testing.
作者信息
Zhang Yu-Qiong, Qiao Long-Wei, Li Wen-Jing, Wang Ya-Nan, Gao Jing-Jing
机构信息
Department of Clinical Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital Suzhou 215104, Jiangsu, China.
Suzhou Key Laboratory of Intelligent Critical Illness Biomarkers Translational Research Suzhou 215104, Jiangsu, China.
出版信息
Am J Transl Res. 2025 May 15;17(5):3649-3657. doi: 10.62347/MTQT6463. eCollection 2025.
OBJECTIVE
The aim of this study was to investigate the effect of platelet distribution width (PDW) on fetal fraction (FF) of cell-free DNA (cfDNA) within non-invasive prenatal testing (NIPT) during pregnancy.
METHODS
The study subjects were pregnant women who voluntarily underwent NIPT and had antenatal examinations at the Affiliated Suzhou Hospital of Nanjing Medical University from January 2016 to January 2021. They underwent routine blood tests before and after NIPT. Univariate and multivariate regression models were used to evaluate the correlation between complete blood count indices and FF.
RESULTS
Multivariate linear regression analysis revealed that hemoglobin (Hb), white blood cell count (WBC), platelet count (PLT), red blood cell distribution width (RDW), PDW, and body mass index (BMI) were negatively correlated with FF, while the interval between the two tests was positively correlated with FF. Notably, PDW (standardized β: -0.097) was the second most significant factor after BMI (standardized β: -0.292). Further grouping by PDW showed that compared to PDW ≤ 12.0 fL, FF decreased by 0.070 (95% CI: -0.111 to -0.030; = 0.001) and 0.191 (95% CI: -0.260 to -0.123; < 0.001) in women with PDW levels of 12.1-15.0 fL and > 15.0 fL, respectively, showing a gradual decreasing trend ( < 0.001).
CONCLUSION
Higher PDW values altered the FF detection, with an observed decrease in FF as PDW levels increased.
目的
本研究旨在探讨孕期无创产前检测（NIPT）期间血小板分布宽度（PDW）对游离DNA（cfDNA）胎儿分数（FF）的影响。
方法
研究对象为2016年1月至2021年1月在南京医科大学附属苏州医院自愿接受NIPT并进行产前检查的孕妇。她们在NIPT前后进行了常规血液检查。采用单因素和多因素回归模型评估全血细胞计数指标与FF之间的相关性。
结果
多因素线性回归分析显示，血红蛋白（Hb）、白细胞计数（WBC）、血小板计数（PLT）、红细胞分布宽度（RDW）、PDW和体重指数（BMI）与FF呈负相关，而两次检测之间的间隔与FF呈正相关。值得注意的是，PDW（标准化β：-0.097）是仅次于BMI（标准化β：-0.292）的第二大显著因素。按PDW进一步分组显示，与PDW≤12.0 fL相比，PDW水平为12.1 - 15.0 fL和>15.0 fL的女性FF分别降低了0.070（95%CI：-0.111至-0.030；P = 0.001）和0.191（95%CI：-0.260至-0.123；P < 0.001），呈现逐渐下降趋势（P < 0.001）。
结论
较高的PDW值改变了FF检测结果，随着PDW水平升高，FF呈下降趋势。
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