Jo YeWon, Song SuMin, Jeong JiHyun, Lee JiHyun, Han Sung Ok, Hyeon Jeong Eun
Department of Next Generation Applied Sciences, Graduate School, Sungshin Women's University, Seoul 01133, Republic of Korea.
Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea.
J Agric Food Chem. 2025 Jul 2;73(26):16444-16453. doi: 10.1021/acs.jafc.5c00566. Epub 2025 Jun 19.
Ulvan, a primary constituent of green algae biomass, has the potential to be used for various biomedical and biotechnology applications. Its biological activity is significantly influenced by its molecular weight. However, the high molecular weight of ulvan poses challenges due to its high viscosity and poor solubility, necessitating an efficient depolymerization process. The ulvan oligosaccharide, produced by decomposing ulvan, not only retains the diverse biological activities of ulvan but also overcomes the issues of low solubility and diminished bioavailability. In this study, ulvan lyase and unsaturated beta-glucuronyl hydrolase were engineered by attaching dockerin to the C-terminus of the enzymes. The resultant ulvan lyase enzyme complex was then reacted with ulvan extracted from biomass to evaluate its depolymerization efficacy. The low-molecular-weight ulvan lysate generated by this enzyme complex showed enhanced prebiotic activity compared to lysate from a single-subunit enzyme. These findings suggest that further decomposition of ulvans via ulvan lyase complex can increase the practical use of ulvan-derived oligosaccharides. Hence, employing electrostatic dockerin-cohesin interactions in seaweed polysaccharide depolymerase complex proves to be effective, offering significant promise for advancing biotechnology research for producing functional oligosaccharides.
岩藻聚糖硫酸酯是绿藻生物质的主要成分，具有用于各种生物医学和生物技术应用的潜力。其生物活性受分子量的显著影响。然而，岩藻聚糖硫酸酯的高分子量因其高粘度和低溶解度而带来挑战，因此需要高效的解聚过程。通过分解岩藻聚糖硫酸酯产生的岩藻寡糖不仅保留了岩藻聚糖硫酸酯的多种生物活性，还克服了低溶解度和生物利用度降低的问题。在本研究中，通过将dockerin连接到酶的C末端来改造岩藻聚糖硫酸酯裂解酶和不饱和β-葡萄糖醛酸水解酶。然后将所得的岩藻聚糖硫酸酯裂解酶复合物与从生物质中提取的岩藻聚糖硫酸酯反应，以评估其解聚效果。与单亚基酶产生的裂解物相比，该酶复合物产生的低分子量岩藻聚糖硫酸酯裂解物显示出增强的益生元活性。这些发现表明，通过岩藻聚糖硫酸酯裂解酶复合物进一步分解岩藻聚糖硫酸酯可以增加岩藻聚糖硫酸酯衍生的寡糖的实际应用。因此，在海藻多糖解聚酶复合物中利用静电dockerin-黏连蛋白相互作用被证明是有效的，为推进生产功能性寡糖的生物技术研究提供了重要前景。
