• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

小鼠全层角膜缘组织中施莱姆管的免疫荧光染色、高分辨率成像及空间投影方案。

Protocol for immunofluorescent staining, high-resolution imaging, and spatial projection of Schlemm's canal in mouse whole-mount corneal limbus tissue.

作者信息

Wang Weifa, Peng Peiyao, Hu Wenfo, Liu Xuyang, Yang Liming, Ju Rong, Zhang Feng

机构信息

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

School of Basic Medicine, Haerbin Medical University, Haerbin 150081, China.

出版信息

STAR Protoc. 2025 Jun 18;6(3):103906. doi: 10.1016/j.xpro.2025.103906.

DOI:10.1016/j.xpro.2025.103906
PMID:40536878
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12212140/
Abstract

Schlemm's canal (SC) is an endothelium-lined conduit with uniquely combined features of blood vessels and lymphatic vessels required for aqueous humor drainage from the eye. Here, we present a protocol for immunofluorescent staining of blood and lymphatic endothelial markers in mouse whole-mount anterior eye cup at different postnatal ages. We describe steps for tissue harvesting, fixation, antibody incubation, mounting, and high-resolution confocal microscopy. This protocol facilitates the understanding of SC formation and functionality at the cellular level. For complete details on the use and execution of this protocol, please refer to Gu et al..

摘要

施莱姆管（SC）是一种内衬内皮的管道，具有独特的血管和淋巴管组合特征，是眼房水引流所必需的。在这里，我们展示了一种在不同出生后年龄的小鼠全层眼前房杯状组织中对血液和淋巴管内皮标记物进行免疫荧光染色的方案。我们描述了组织采集、固定、抗体孵育、封片和高分辨率共聚焦显微镜检查的步骤。该方案有助于在细胞水平上理解施莱姆管的形成和功能。有关该方案的使用和执行的完整详细信息，请参考顾等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/f9860d5e094d/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/70490c5e3b84/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/77d87811a6a1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/942fec8c5b5e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/931563896304/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/abab2fa6774f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/188e4299f562/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/f9860d5e094d/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/70490c5e3b84/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/77d87811a6a1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/942fec8c5b5e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/931563896304/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/abab2fa6774f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/188e4299f562/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd1e/12212140/f9860d5e094d/gr6.jpg

相似文献

1
Protocol for immunofluorescent staining, high-resolution imaging, and spatial projection of Schlemm's canal in mouse whole-mount corneal limbus tissue.小鼠全层角膜缘组织中施莱姆管的免疫荧光染色、高分辨率成像及空间投影方案。
STAR Protoc. 2025 Jun 18;6(3):103906. doi: 10.1016/j.xpro.2025.103906.
2
Conjunctival autograft for pterygium.翼状胬肉的结膜自体移植术。
Cochrane Database Syst Rev. 2016 Feb 11;2(2):CD011349. doi: 10.1002/14651858.CD011349.pub2.
3
Anti-vascular endothelial growth factor for neovascular glaucoma.抗血管内皮生长因子治疗新生血管性青光眼。
Cochrane Database Syst Rev. 2023 Apr 3;4(4):CD007920. doi: 10.1002/14651858.CD007920.pub4.
4
Protocol for the visualization of bacteria in the tick gut using whole-mount fluorescence in situ hybridization.使用全组织荧光原位杂交技术观察蜱肠道内细菌的实验方案。
STAR Protoc. 2025 May 6;6(2):103814. doi: 10.1016/j.xpro.2025.103814.
5
Interventions for recurrent corneal erosions.复发性角膜糜烂的干预措施。
Cochrane Database Syst Rev. 2018 Jul 9;7(7):CD001861. doi: 10.1002/14651858.CD001861.pub4.
6
Chlorhexidine mouthrinse as an adjunctive treatment for gingival health.洗必泰漱口水作为牙龈健康的辅助治疗方法。
Cochrane Database Syst Rev. 2017 Mar 31;3(3):CD008676. doi: 10.1002/14651858.CD008676.pub2.
7
Rho kinase inhibitor for primary open-angle glaucoma and ocular hypertension.Rho 激酶抑制剂治疗原发性开角型青光眼和高眼压症。
Cochrane Database Syst Rev. 2022 Jun 10;6(6):CD013817. doi: 10.1002/14651858.CD013817.pub2.
8
Adjunctive steroid therapy versus antibiotics alone for acute endophthalmitis after intraocular procedure.辅助性类固醇疗法与单纯抗生素疗法治疗眼内手术后急性眼内炎的对比
Cochrane Database Syst Rev. 2017 Feb 22;2(2):CD012131. doi: 10.1002/14651858.CD012131.pub2.
9
Electronic cigarettes for smoking cessation.电子烟戒烟。
Cochrane Database Syst Rev. 2021 Sep 14;9(9):CD010216. doi: 10.1002/14651858.CD010216.pub6.
10
Adjunctive steroid therapy versus antibiotics alone for acute endophthalmitis after intraocular procedure.眼内手术后急性眼内炎中辅助性皮质类固醇治疗与单独使用抗生素的比较。
Cochrane Database Syst Rev. 2022 Jun 6;6(6):CD012131. doi: 10.1002/14651858.CD012131.pub3.

本文引用的文献

1
Macrophage-induced integrin signaling promotes Schlemm's canal formation to prevent intraocular hypertension and glaucomatous optic neuropathy.巨噬细胞诱导整合素信号促进房水流出通道的形成，以预防眼内高压和青光眼视神经病变。
Cell Rep. 2024 Feb 27;43(2):113799. doi: 10.1016/j.celrep.2024.113799. Epub 2024 Feb 16.
2
Morphological Analysis of Schlemm's Canal in Mice.小鼠施莱姆管的形态学分析
Methods Mol Biol. 2018;1846:153-160. doi: 10.1007/978-1-4939-8712-2_10.
3
VEGF-C-dependent stimulation of lymphatic function ameliorates experimental inflammatory bowel disease.
血管内皮生长因子C依赖性淋巴功能刺激改善实验性炎症性肠病。
J Clin Invest. 2014 Sep;124(9):3863-78. doi: 10.1172/JCI72189. Epub 2014 Aug 8.
4
Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process.施莱姆管是一种独特的血管，具有血管和淋巴管表型的组合，通过一种新的发育过程形成。
PLoS Biol. 2014 Jul 22;12(7):e1001912. doi: 10.1371/journal.pbio.1001912. eCollection 2014 Jul.
5
Effect of fixation pressure on juxtacanalicular tissue and Schlemm's canal.固定压力对近小管组织和施莱姆管的影响。
Invest Ophthalmol Vis Sci. 1996 Jan;37(1):114-24.
6
Pressure-dependent changes in configuration of the endothelial tubules of Schlemm's canal.施莱姆管内皮小管构型的压力依赖性变化。
Am J Ophthalmol. 1974 Oct;78(4):630-8. doi: 10.1016/s0002-9394(14)76301-9.