腺相关病毒介导的基因递送加速小鼠胰腺癌发生的实验方案
Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice.
作者信息
Li Hongzhen, Shi Zhao, Shen Shanshan, Kong Bo
机构信息
Department of Gastroenterology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, China; Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany.
Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany; Department of Gastroenterology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, China.
出版信息
STAR Protoc. 2025 Jun 18;6(3):103907. doi: 10.1016/j.xpro.2025.103907.
Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48; LSL-Kras (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facilitate the progression from acinar cells through acinar-to-ductal metaplasia (ADM) to pancreatic intraepithelial neoplasia (PanIN). We describe steps for AAV production, preparing KC mice, intra-pancreatic AAV injection, and analysis. This protocol allows the investigation of early events of pancreatic carcinogenesis in a spatially and temporally controlled manner. For complete details on the use and execution of this protocol, please refer to Li et al..
引入额外的基因修饰或环境刺激会加速p48；LSL-Kras（KC）小鼠模型中的早期胰腺癌发生。在此，我们展示了一种利用腺相关病毒（AAV）介导的基因递送方法，专门作用于胰腺，以促进从腺泡细胞经腺泡-导管化生（ADM）发展到胰腺上皮内瘤变（PanIN）的过程。我们描述了AAV生产、制备KC小鼠、胰腺内AAV注射及分析的步骤。该方法允许以空间和时间可控的方式研究胰腺癌发生的早期事件。有关本方法使用和实施的完整详细信息，请参考Li等人的研究。
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